Determination of romanian alfalfa crude protein and crude fiber contents aswell as in vitro organic matter digestibility by nir spectrometry

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Laura M. DALE, André THEWIS, Ioan ROTAR, Christelle BOUDRY, Roxana VIDICAN, Anamaria MALINAS, Vasile FLORIAN, Bernard LECLER, Richard AGNEESSENS, Juan A. FERNÁNDEZ PIERNA, Vincent BAETEN

Alfalfa (Medicago sativa L.) is a high quality forage which has been used worldwide. The superiority of alfalfa lies in its high yield, high protein content and high digestibility. The aim of this study was to develop a simple, fast and non­destructive method, named Near Infrared Spectroscopy (NIRS) to determinate alfalfa quality. To realize this study, alfalfa samples were obtained from Manaútur Experimental Station – Farm Cojocna in 2008–2009, in one experiment carried out using randomization blocks design with two experimental factors (mineral fertilization and period of harvest). Alfalfa quality was first determinated on 48 samples by classical analyses: crude protein CP (AOAC, 1990), crude fiber CF (Fiber Cap, FOSS, DK) and in vitro organic matter digestibility OMDrt (DeBoever, 1986). Then the samples were scanned by NIRS. Calibration models were performed by PerkinElmer Spectrum Quant + 4.21 program (USA) on the 48 samples determinated by classical analysis. The results showed fully confirmed by acceptable coefficients of determination and standard error of cross-validation (R2=0.96 for CP, 0.94 for CF, 0.98 for OMDrt and SECV=0.77 for CP, 1.35 for CF, 1.13 for OMDrt). Successful results for prediction of other 176 alfalfa samples were then obtained using these calibration models: SEP=0.869 for CP, 1.058 for CF, 1.058 for OMDrt). The highest CP and OMDrt values of alfalfa were obtained in bud stage (22.0% and 66.0% respectively). While for CF, the highest content was registered in the seed formation stage (46.0%). The NIRS technique offers us the possibility to determinate rapidly and easily Romanian alfalfa important parameters, but the system could also be used for the determination of other constituents.

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M. Dale L., Thewis A., Rotar I., Boudry C., Vidican R., Malinas A., Florian V., Lecler B., R.Agneessens, A. Fernández Pierna J., Baeten V., 2012, Determination of romanian alfalfa crude protein and crude fiber contents as well as in vitro organic matter digestibility by nir spectrometry. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 57-62.

 

Determination of Zerdava dog (Kapi Kopegi) raised in northeast of Turkey

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Orhan YILMAZ, Mehmet ERTUGRUL

This is the first document on Turkish Zerdava dogs raised in northeast of Turkey. This study was carried out to define the morphologic traits of the Turkish Zerdava dogs raised in east of Turkey comparing with some other native dog breeds of Turkey. To this end, a total of 39 (19 male and 20 female) dogs was analyzed using the Minitab 15 statistical software program using ANOVA and Student’s t-Test. Descriptive statistics and comparison results were for height at shoulders 51.2±0.35, height at rump 51.6±0.28, body length 56.3±0.35, heart girth circumference 50±1.43, chest width 25.6±0.22, cannon circumference 9.4±0.14, and head length 19.4±0.17 cm respectively. The overall results of the study demonstrated that Turkish Zerdava dogs had a very close resemblance to the Turkish Kangal (Karabash) and Akbash Shepherd dogs related with body measurements. The Zerdava dogs reach mature body weight and size at around 18 months of age.

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Yilmaz O., Ertugrul M., 2012, Determination of Zerdava dog (Kapi Kopegi) raised in northeast of Turkey. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 258-261.

 

Effect of feed on the basis of soybean in pig nutrition

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Dragan MILIĆ, Vladislav STANAĆEV, Ana MARJANOVIĆ - JEROMELA, Vidica STANAĆEV, Nikola PUVAČA, Sava ZARIĆ

This paper is a review of summarizing the results of researchers who have studied the effect of soybean meal and soybean grits in the diet of certain categories of pigs. As for the sows and fattening pigs soybean meal may be the only protein feed in the diet, which meets the needs of these groups in proteins, typically without correction of amino acids with synthetic amino acids. If the meal is properly heat treated, diet of fattening pigs is possible without the use of fish meal, and when that does not deteriorate the basic parameters of production. Soybean meal in addition to high quality protein and contains significant amounts of fat, which is of particular importance in the nutrition of young pigs that have not yet developed a system for efficient enzymatic digestion of cereals, the only source of energy in the diet. The use of soybean grits increased daily gain of piglets and improves feed efficiency compared to the group in which was used powdered milk. The maximum level of soybean grits in the diet of piglets is 30% and soybean meal in the diet of fattening pigs and sows 30%.

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Milić D., Stanaćev V., Marjanović – Jeromela A., Stanaćev V., Puvača N., Zarić S., 2012, Effect of feed on the basis of soybean in pig nutrition. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 67-72.

 

Effect of low density lipoproteins in extender on freezability and fertility of egyptian buffalo bull semen

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by El-Sharawy, M. E.; El-Shamaa, I. S.; Ibrahim, M.A.R.; Abd El-Razek, I.M.; El-Seify, E.M.

Semen from three Egyptian buffalo bulls was collected once weekly and ejaculates with more 75% progressive motility and more 85 % normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bulls effect. Seven extenders were used: Tris 20 % egg yolk extender with 7 ml glycerol as a control (T1), and substitution of whole egg yolk with 4, 6, 8, 10, 12 and 15 % low density lipoprotein (LDL), T2 – T6, respectively. Semen was diluted to 80 x106 sperm/ml, packaged into 0.25 ml straws, cooled, held at 5.C for 4 h, and then frozen in liquid nitrogen (LN) and stored at -196.C for at least one month. Sperm progressive motility, intact acrosome and plasma membrane integrity were assesd at post dilution, equilibration, post-thawing (at 37.C for 30 sec.) and after 30 days storage in LN. This study reveled that LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buffalo spermatozoa than whole egg yolk extender. Sperm progressive, intact acrosome and plasma membrane integrity were much higher (P < 0.05) in the 12% LDL extender (63.3, 77.17 and 71.3% respectively) vs. 35, 40.8 and 34.7% in the control 20% EY extender at post-thawing process, respectively. Fertility rates were higher in extender containing 12% LDLs compared with the control (72.7% vs. 50%, respectively). It was concluded that LDL (12%) in extender improved the freezability and fertility of buffalo bull spermatozoa.

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El-Sharawy M. E., El-Shamaa I. S., Ibrahim M.A.R., Abd El-Razek I.M., El-Seify E.M., 2012, Effect of low density lipoproteins in extender on freezability and fertility of egyptian buffalo bull semen. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 114-120.

 

Effect of thawing methodson physical characteristic and chemical composition ofrib eye meat ongole crossed

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Kusmajadi SURADI, Azeisha Diena RAHMANI, Maria Sri HARTATI, Nani DJUARNANI

The research was aim to know effect of thawing methods on physical characteristic and chemical composition of rib eye meat Ongole Cross, and to know method best thawing based physical characteristic of and chemical composition. Research is conducted experimentally used Completely Randomized Design (CRD) with four treatments thawing, that is: refrigeration temperature (5-70C), room temperature (27-300C), running water at room temperature (22-240C), and warm water temperature (50-550C) each treatment was repeated five times. Analysis of variance conducted to determine the effect thawing methods on the phyisical and chemical composition of rib eye meat Ongole Crosses, while to know difference between the treatment used Duncan’s test. The result of statistic analysis and discussion indicated that the method of thawing significant effect (P<0,05) of physical characteristic and chemical composition of rib eye meat Ongole Crosses. The method of thawing with refrigeration temperature has the best effect on chemical composition and physical characteristicof rib eye meat Ongole Cross.

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Suradi K., Rahmani A. D., Hartati M. S, Djuarnani N., 2012, Effect of thawing methodson physical characteristic and chemical composition of rib eye meat ongole crossed. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 290-295.

 

Effect of use of premix in dairy cows

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Ylli BIÇOKU, Valbona YLLI

The aim of our study was to analyze the effect of using premix on milk production and reproduction indicators. The study was conducted for a period of 12 months, in 10 small (up to 4 cows) and medium farms (about 6 cows), which are breeding Holshtein breed, in Lushnja and Fier districts. The data are showing that, cows that have received throughout lactation premix have produced 162 liters of milk more than those not treated with premix. Also, the days open period was 21 days shorter than cows not treated with premix and the insemination index was improved. Farmers note that cows consume premix are vital and have better appetite than the group of cows that do not take premix. Statistical data processing was done with Statgraphics Centurion XVI.

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Biçoku Y., Ylli V., 2012, Effect of use of premix in dairy cows. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 37-41.

 

Effects of cooking methods on the heavy metal concentrations in the fish meatoriginating from different areas of Danube river

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Cristiana DIACONESCU, Laura URDEȘ, Ștefan DIACONESCU, Dana POPA

Fish is a major source of mineral but it can also contain heavy metals. The present study has shown that the heavy metal concentration in fish meet can be affected by processing or cooking methods. Chromium (Cr), nickel (Ni), cadmium(Cd) and lead (Pb) were analyzed in cooked fish meat(4 sweet water fish species :bream, mackerel, carassius and perch, originated from the area of Sulina Arm and auxiliary canals). Various cooking methods were used such as grilling, frying, microwaving and baking. The Pb concentrations of the samples varied between 0.08 and 0.14 mg/kg . There were no significant differences (P > 0.05) in Pb concentrations between the raw, grilled, fried, baked and microwave-cooked fish. Ni contents decreased in grilled fish. Cr concentrations in grilled and microwave-cooked fish decreased significantly (P < 0.05).Cadmium was found only in fried meat fish. The results show that the grilling, microwave cooking and baking are suitable methods.

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Diaconescu C., Urdeș L., Diaconescu Ș., Popa D., 2012, Effects of cooking methods on the heavy metal concentrations in the fish meat originating from different areas of Danube river. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 265-267.

 

Effects of feeding different levels of guar meal on performance and bloodmetabolites in Holstein lactating cows

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Morteza Salehpour, Khosro Qazvinian and Vasco A. P. Cadavez

A study was carried out to determine the effects of using different levels of Guar meal on performance and blood metabolites in Holstein lactating cows. Sixteen lactating Holstein cows(DIM=95±10)were used in Latin square design with 4 block and 4 repeats. Animal in each group fed 1 of 4 experimental rations. Diets contain 0, 50, 75 and 100 percentage cottonseed meal were replaced with gaur meal. Diets were similar as NEL and crude protein (Iso caloric and iso nitrogenous) on dry basis. Cows were fed with total mixed ration individually. Dry matter intake and milk yields were higher for cows fed with 0% guar meal and lowest for 100 cottonseed meal replaced by guar meal, but no significant difference were found among FCM 4%.Milk fat and protein percentage and yields were highest for 50 % Guar meal, but no significant difference was found between milk lactose and calcium. Milk Urea Nitrogen and blood urea nitrogen were not significantly affected by experimental diets.

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Salehpour M., Qazvinian K., A. P. Cadavez V., 2012, Effect sof feeding different levels of guar meal on performance and blood metabolites in Holstein lactating cows. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 73-77.

 

Essay on estimation of undemonstrative spare outputs disclosed by reproduction biotechnologies in sheep breeding

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Marcel Theodor PARASCHIVESCU, Alexandru ȘONEA, Alexandru BOGDAN, Marcel PARASCHIVESCU, George Florea TOBĂ

Among genetic species of farm animals sheep has the highest artificial biodiversity. There are plenty of breeds due to the many kinds of biological production of human interest and to the large areas from plains to mountains and fro Equator to Poles engaged in sheep breeding. Sheep are producing wool (thin, meddle or thick), lambs, mouton (lean or fat), milk, pelts, furs or leather, each of them acting as single selection criterion or as component of selection indexes. Each kind of resulted product has its own commercial value and its way of appreciation it. In many cases the commercial value of sheep breeding is related to the ewes’ fertility. On the other hand ewes have seasonal sexual activity including a longer or shorter period of anoestrus when produce nothing related to their fertility. Biotechnological means as artificial insemination, estrus induction, arouse of ovulation rate, embryo transfer, MOET, embryo cloning, in vitro fertilization, transgenice engineering are able to influence the economic efficiency of sheep breeding. The present essay is configuring modalities of estimating the increase of economic value of sheep products when research programs of developing reproductive biotechnologies in ewes implemented in sheep farming.

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Paraschivescu M. T., Șonea A., Bogdan A., Paraschivescu M., Tobă G. F., 2012, Essay on estimation of undemonstrative spare outputs disclosed by reproduction biotechnologies in sheep breeding. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 134-139.

 

Genetic investigations using immuno-biochemical markers in a black spotted cattle population

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Nicoleta IȘFAN, Tomița DRĂGOTOIU, Monica MARIN, Ștefania DOROBANȚU, Dana POPA, Carmen Georgeta NICOLAE

The study of the genetic markers and identifying new markers involves an increasing number of research projects in the fields of genetics of immunology, biochemical genetics, molecular genetics, quantity genetics and the genetic improvement of animals.Having considered the importance of genetic polymorphism of biochemical structures we considered a study of the genetic characterization of a sample in a cattle population, based on the information offered by the genetic polymorphism at hemoglobin and transferrine loci and the analysis of the serum. Two phenotypic category, hemoglobin A (79,4%) and AB (20,6%), has been identified for hemoglobin locus. Five categories of individuals, homozygous for TfA/TfA (2.9%), TfD/TfD (58,8%) and heterozygous for TfA/TfD (20.6%), TfA/TfE (5,9%), TfD/TfE (11,8%) have been identified on seric transferine loci; determined of existence of interest locus for three kinds of genes TfA, TfD and TfE with codominant effect. Blood factors were described in order to use them as genetic markers with the purpose of determine the homogenity or heterogenity degree for two cattle population.Serologic relations were observed between the factors of B system. The most relevant was the complex BGK. These tree factors were observed in five different combinations, namely BGK, BG, B, G, and their total absence.

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Ișfan N., Drăgotoiu T., Marin M., Dorobanțu Ș., Popa D., Nicolae C. G., 2012, Genetic investigations using immuno-biochemical markers in a black spotted cattle population. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 15-19.

 


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