Research on sperm quality of north american sturgeon species Polyodon spathula

Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Mioara COSTACHE, Cecilia BUCUR, Daniela RADU, Daniel OPREA, Mihail COSTACHE, Carmen Georgeta NICOLAE

The paper presents research on sperm quality and affecting factors in male of North American sturgeon species Polyodon spathula recently acclimated in Romania. Quality assessment was performed by microscopic and macroscopic analysis of sperm from a total of 5 males used in artificial spawning experiments in 2012. Microscopic analysis was to determine the concentration of sperm by haemocytometer method, sperm shaped and size determination using electronic microscopy and identification of specific staining, motility determination using dark field microscopy, testing the viability of sperm after sperm activation. Sperm quality parameters were interpreted by correlation with following factors: hormonal stimulation, the spawning season, temperature, extenders properties, short or long term storage of sperm, the biology of spawning (age, weight, length), health and wellness condition.

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Costache M., Bucur C., Radu D., Oprea D., Costache M., Nicolae C. G., 2012, Research on sperm quality of north american sturgeon species Polyodon spathula. Scientific Papers. Series D. Animal Science, Vol. LV, ISSN-L 2285-5750, 306-311.

 


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