Published in Scientific Papers. Series D. Animal Science., Vol. LVI
Written by Valentina CEBOTARI, I. TODERAS, I. BUZU1, V. RUDIC
The goal of the work was to determine the biostimulation effect of a nutritional supplement of sugar syrup enriched with biomass extract of cyanobacteria Spirulina platensis strain, grown on remedy of chromium alum and potassium, on vital activities functions of Apis mellifera bee colonies. In special experiences has been tested in bees food, an energymineralo- protein nutritional supplement „Apispir+Cr”, composed of sugar syrup in proportion 1:1, enriched with biomass extract of cyanobacteria Spirulina platensis strain, grown in the presence of coordinate compound of chromium and potassium alum-KCr (SO4) 2·12H2O administered in nutrient medium componence, in the amount of 30...35 mg/L in the first three days of cultivation. The extract of the cyanobacteria Spirulina platensis stem biomass has been obtained by repeated extraction of biologically active substances with alcohol solution and solution of NaOH, by centrifuging, by combinig the supernatants and by making the dialysis up to pH 7.5...8.5 with getting the final extract. Nutritional supplement 'Apispir+Cr' was tested on two similar batches with bee colonies, with 10 families in each batch, including:-Ist batch-witness, where the bees were fed with sugar syrup 1:1, in amount of 100...130 mL to a frame with bees, at every 2 days during two weeks;-II batch-experimental, where bees were fed with sugar syrup 1:1, in the same amount, enriched with the 'Apispir+Cr' supplement under 500:1, and syrup with extract. In early may, before the base picking, were examined the main morphoproductive characters and qualities of bee colonies. Test results have shown that feeding the bees with a nutritional supplement 'Apispir+Cr' in early spring, during the poor harvest in nature, contributed to a significant increase in the value of the main morphoproductive characters of bee colonies. Thus, bee colonies from experimental batch, who received in food the nutritional supplement enriched with 'Apispir+Cr', exceeded their fellows: after the prolificity – with 1390 eggs/24 hours, or with 74.4% (P < 0.001), quantity of covered brood-with 167 hundreds of cells, or with 74.5% (P < 0.001), the power of the family-with 0.56 kg or 25.3% (P < 0.001), resistance to disease-with 10.8 percentage, or 13.4% (P < 0.001), the amount of wax accumulated in nest-with 1.10 kg, or with 8.,6% (P < 0.05), the amount of honey in the nest-2.44 kg, or 90.0% (P < 0.001), the quantity of pasture accumulated in the nest-with 36.4 hundreds of cells, or with 106.7% (P < 0.001).
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