ISSN 2285-5750, ISSN CD-ROM 2285-5769, ISSN-L 2285-5750, ISSN Online: 2393 – 2260
 

Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Săndița PLĂCINTĂ, Mirela CREȚU, Victor CRISTEA, Iulia Rodica GRECU, Anca Nicoleta CORDELI (SAVESCU), Elena SÎRBU

A feeding trial was conducted to evaluate the effects of dietary vitamin C (L-ascorbic acid, AA) levels on haematological profile and some biochemical indices of European catfish, reared in a recirculating aquaculture system. A basal commercial diet (40% crude protein and 11.5% lipids) was used as a control (D0), and three other diets were prepared by supplementing the feed with 50 mg AA kg-1 diet (D1), 100 mg AA kg-1 diet (D2) and 150 (D3) mg AA kg-1 diet. At the end of the feeding trial, blood samples were taken in order to analyse the haematological profile and serum biochemical parameters. Significant differences (p˂0.05) were recorded among experimental diets in the numbers of red blood cells (RBC), haematocrit (Hct), the mean corpuscular volume (MCV), and the mean corpuscular haemoglobin concentration (MCHC). Regarding the serum glucose, serum total protein, and the concentration of immunoglobulin (IgM) it was observed an insignificant increase (p˃0.05) with the increasing of the level of vitamin C. In conclusion, supplementation of vitamin C in the diet of Silurus glanis led to good results on fish welfare.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 2
Written by Tsvetelina NIKOLOVA

Cultivation of mulberry silkworm is economically efficient branches of agriculture. It originated in ancient times. The strong dependence on nutritional requirements and especially the seasonality and range of distribution of mulberries are limiting factors for the cultivation of mulberry silk butterfly larvae. Due to its peculiarities, Morus alba develops well and yields high leaf yields at certain seasons of the year in our country. The resulting leaf mass is of high nutritional value. The artificial food for Bombyx mori L. enables cultivation during any season of the year, regardless of the external climatic conditions. Some plant extracts are nutritional stimulants and improve nutritional intake, growth and even disease resistance. The purpose of this study is to test a hybrid I1xBB1xH2xHB2 created in the Scientific Center on Sericulture, Vratsa, Bulgaria on susceptibility to artificial food with added extract of Tribulus Terrestris L. as a stimulant. Tracking the most important biological, reproductive and technological features of silk larvae and butterflies. Artificial food was widely accepted by Bombyx mori L. Higher values were observed in the experimental groups fed with artificial food and added extract of Tribulus terrestris L. We observed the larval growth rate and vitality.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 2
Written by Ștefan-Mihai PETREA, Ira-Adeline SIMIONOV, Alina MOGODAN, Aurelia NICA, Dragoș CRISTEA, Mihaela NECULIȚĂ

The meat nutritional value is an important factor that influence consumer preferences for certain fish species. Thus, the aim of this study is to identify the influence of feeding rate on stellate sturgeon meat biochemical composition, assessed by determining the percentage of dry matter, crude fat, crude protein and ash. Two feeding rates were tested (F1 - 1% BW, respectively F2 - 2% BW), in replicate. The biochemical evaluation of stellate sturgeon meat revealed better values in terms of crude protein content at F2 duplicate (17.86±0.14 %, respectively 17.70±0.17%), compared to F1 experimental variant duplicate (16.71±0.2%, respectively 16.78±0.18%). Also, F2 registered a higher crude fat (1.32±0.04%, respectively 1.36±0.03) and ash content (1.69±0.06%, respectively 1.77±0.09), compared to F1 (crude fat: 1.21±0.04%, respectively 1.27±0.05%; ash: 1.33±0.10%, respectively 1.39±0.11%). The water-to-protein ratio indicate a high nutritional value for meat of stellate sturgeon exemplars, reared by applying a feeding rate of 2% BW (4.31±0.15, respectively 4.35±0.17 at F2 duplicate, compared to 4.71±0.11, respectively 4.67±0.10 at F1). As a conclusion, the use of 2% BW feeding rate for rearing stellate sturgeons in a recirculating aquaculture system (RAS) assures a superior protein and fat retention and improves protein use efficiency (PUE).

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 2
Written by Marie NAJOAN, Betty BAGAU, Fenny WOLAYAN, Florencia SOMPIE, Youdhi KOWEL

Although its protein content is less than that of conventional ingredients commonly used as protein sources, copra meal represents the largest quantity of locally available feed protein in many tropical areas, such as Indonesia. It is, therefore, important that information about the nutritional value of copra meal is available. The present study was conducted to test the effect of inclusion of copra meal in growing pigs diets on nutrient digestibility. Twenty-four castrated Landrace Yorkshire pigs (3.5 – 4.0 months of age, ranged from 33 – 40 kg of body weight) were kept in individuals pens. Four treatments were arranged as follow: R1 = 95% basal diet + 5% copra meal; R2 = 90% basal diet + 10% copra meal; R3 = 85% basal diet + 15% copra meal; R4 = 80% basal diet + 20% copra meal, with six replications as blocks based on body weight. The present study was arranged in a 4 x 5 Completely Randomized Block Design with four treatments and five blocks. After a significant F test, Tukey’s test (where necessary) was used to inspect differences among treatment means. Differences between treatment means were considered significant when P < 0.05. Variables measured were: daily feed and water consumption, nutrient digestibility (energy, crude protein, fat, crude fiber, calcium and phosphorus). The result showed that there was no significant effect (P>0.05) of copra meal inclusion in diet on feed and water consumption, as well as nutrient digestibility (energy, crude protein, fat, crude fiber, calcium and phosphorus). It can be concluded that inclusion of copra meal up to 20% in growing pigs diets did not affect nutrient digestibility as well as performance of the pigs. Further study on higher level of copra meal inclusion in pig diets is needed.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 1
Written by Alina MOGODAN, Ștefan-Mihai PETREA, Ira-Adeline SIMIONOV, Aurelia NICĂ, Dragoș CRISTEA, Mihaela NECULIȚĂ

In the present study, three phytobiotics were added to the basal diet of Oreochromis niloticus reared in a recirculating aquaculture system. Therefore, was investigated the influence of phytobiotics on hematological profile, some blood biochemical indices and leukocyte reaction. The experiment was carried out during 12 weeks. The experimental variants were: V1 - control; V2 - 1% Rosmarinus officinalis/kg feed; V3 - 1% Hippophae rhamnoides/kg feed and V4 - 1% Zingiber officinale/kg feed. The results of some parameter of hematological profile showed that supplemented diet with sea buckthorn and ginger exhibited significantly (p<0.05) lowest values of red blood cells count, white blood cells count, absolute number of lymphocytes and neutrophils, relative number of neutrophils. Regarding to blood biochemical analysis was observed a significant (P<0.05) reduction of plasma cortisol concentration and a slight decrease (P>0.05) of glucose concentration in V3 variant. Also, only the sea buckthorn (V3) and ginger (V4) showed an immunomodulatory effect during the experiment because they determined the intensifying the lysozyme activity. In conclusion, dietary supplementation with sea buckthorn and ginger reduced the technological stress and improved the immunity of Nile tilapia.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by John ABIONA, Fatimah DUROSINMI, Yemisi AYO-AJASA, Muhammed ONAGBESAN

The effect of shell injury on growth and haemocyte concentration were evaluated in this study. Thirty-two (32) snails between 130-180g were randomly divided into four (4) treatments with eight (8) replicate each. The four treatments include: T1 (control), T2, (1 cm shell damage) T3 (2 cm shell damage) and T4 (3 cm shell damage). Haemolymph was collected on weekly basis for four weeks. Parameters monitored were total haemocyte count and shell growth. Result showed that shell injury/damage had significant effect (P<0.001) on total haemocyte count and shell growth. It can be concluded from this study that shell injury had influence on immune response of the animal, although compensatory growth was recorded after week four of the experiment. It can be recommended from this study that irrespective of level of shell damage used in this study, adequate attention should be given not to kill the animal as the process compromise the total haemocyte count which is responsible for immune defense of the animal. It is therefore recommended that adequate care and proper hygiene must be maintained in other not allow opportunistic infection since immune cells (haemocytes) are compromised in other not cause economic loss due to unforeseen mortality.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 1
Written by Marie NAJOAN, Fenny WOLAYAN, Florencia SOMPIE, Betty BAGAU

The purpose of this research was to determine the substitution of goroho banana (Musa acuminafe sp.) stem meal fermented with Trichoderma viride on blood lipid profiles and meat quality of broiler chicken. One hundred and twenty day old chick CP 707 divided into 20 unit cages, each unit consisted of 6 chickens. Complatelly Randomised Design (CRD) was used in this research with 4 treatments and 5 replications. The treatments were: R0 = 100% corn without goroho banana stem meal fermented, R1 = 95% corn + 5% goroho banana stem meal fermented, R2 = 90% corn + 10% goroho banana stem meal fermented and R3 = 85% corn + 15% goroho banana stem meal fermented. Parameter measured were blood lipide profiles (triglyceride, total cholesterol, HDL, LDL) and meat cholesterol. The results showed that substitution of goroho banana stem meal fermented had a significant effect (P<0.05) on a decreased in blood trigliseride, blood cholesterol and meat cholesterol, R3 has significant different (P<0.05) lowest compared to the other treatments (R0, R1 and R2). On the other hand there were no significant effect (P>0.05) on blood HDL and LDL among the treatments. It can be concluded that goroho banana (Musa acuminafe sp.) stem meal fermented with Trichoderma viride can be substituted up to 15% corn meal in ration which improved the meat quality.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 1
Written by Meity SOMPIE, Siswosubroto SURTIJONO, Meilani Rita TINANGON

The quality of gelatin depends on its physichochemical properties and manufacturing method. The process of gelatin required the extraction step to improve the quality of gelatin. The aimed of this study was to research the effect of temperature extraction and extraction time on the characteristics of gelatin produced from pigskin. This study used Completely Randomized Design (CRD) with two factors 3x3 and three replicates of treatments. The first factor was temperature extraction with 3 levels (50oC, 60oC and 70oC). The second factor was extraction time consisting of 3 levels (3 hours, 6 hours and 9 hours). The result showed that the interaction of the extraction temperature and extraction time had significant effect (P<0.05) to content of protein, gel strength, yield and viscocity from gelatin of pigskin. The highest amino acid content was glycine. It was concluded that the use of extraction temperature 600C and time of extraction 6 hours was the best characteristics of pigskin gelatin.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Sonya IVANOVA, Tanya NIKOLOVA

The aim of the study was to investigate the effect of the probiotic preparation Baykal EM-1 on the growth performance, blood parameters and behaviour of weaned pigs. А research experiment with a total of 96 growing pigs, divided into two groups - control (48) and experimental (48), fed with the probiotic Baykal EM-1 in the amount of 10 ml/kg feed, was carried out. The indicators average live weight, feed consumption, daily gain, feed conversion, blood parameters and behaviour of pigs, were studied. The following conclusions were made: The addition of Baykal EM-1 (10 ml/kg feed) in weaned pig diets improved the average daily gain by 11% (P = 0.031). The higher number of leukocytes and lymphocytes in pigs from the experimental group (P<0.001), compared to the control group, may be an indicator of better health and higher immunity. A trend for better comfort in animals with the microbiological supplement in the feed, contributing to the better absorption of nutrients, has been established. The results obtained in this study show that the combination of probiotics Baykal EM-1 has the potential for use as a dietary supplement in weaned pigs.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Abun ABUN, Denny RUSMANA, Tuti WIDJASTUTI, Kiki HAETAMI

One of the efforts to improve feed quality is by using BLS (Bacillus licheniformis., Lactobacillus spp. andSaccharomyces cerevisiae) microbial services from the shrimp waste substrate, hereinafter referred to as PrebioticsBLS.BLS microbes are probiotic organisms that can improve the performance of livestock effectively (growth-promoter).PrebioticsBLS is expected to function as an emulsifier in increasing nutrient absorption and metabolism so that it iseffective in converting feed proteins into growth in Indonesia local chickens. The aim of the study was to determine theresponse of PrebioticsBLS in feed to the performance of Indonesia local chickens. The study used 120 local one-day oldchickens which were divided into 24 cage units, each cage unit consisted of 5 chickens, and kept for eight weeks. The experimental design used was a completely randomized design, consisting of 6 feed treatments (R0 = basal feed / CP15%; R1 = basal feed + 1.0% PrebioticsBLS; R2 = basal feed + 1.5% PrebioticsBLS; R3 = basal feed + 2.0%PrebioticsBLS; R4 = basal feed + 2.5% PrebioticsBLS; and RS = standard feed / CP 18%), each treatment was repeatedfour times. The variables observed were feed consumption, protein consumption, body weight gain, and proteine fficiency ratio. Data obtained from the results of the study were analyzed using analysis of variance, and differences between treatments were tested using Duncan's Multiple Distance Test. The experimental results showed that PrebioticsBLS (based on shrimp waste) can be used as a feed supplement in the local chicken feed formula, and the use of PrebioticsBLS at the level of 1.5-2.0% in feed (R4) is equivalent to the standard ration (RS / CP 18%).

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© 2019 SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE. To be cited: SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE.

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