Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Daniela-Mihaela GRIGORE, Narcisa Elena POGURSCHI, Ionuţ RANGA, Narcisa BĂBEANU
Vitamin A is an essential nutrient, for both production and reproduction of farm animals, however, most animals are unable to synthesize de novo precursors of vitamin A (β-carotene), and dietary supplementation is mandatory. The current paper aims to evaluate the analytical parameters and to improve the method efficiency for determining β-carotene content in red yeast and the internal laboratory validation protocol. Our method stands for the ultraviolet-visible spectrophotometric uses, having the Lambert-Beer law as the basis. To determine the β-carotene content, dimethyl-sulfoxide was employed as a solvent, calibration curve, and visible spectra were evaluated (300-900 nm). The linearity of beta carotene measured at 465 nm using the UV-Vis method linearity range was 6.1-36.6 µg/ml, R2=0.997, LOD = 5.39 µg/ml, LOQ = 16.32 µg/ml, SD less than 5% and RSD in between 1-15%. In conclusion, the β-carotene spectroscopic method determination is a cheap, and efficient method, suitable for β-carotene determination and retinol and retinoic acid estimation for nonconventional feed additives such as yeasts.
[Read full article] [Citation]
