Published in Scientific Papers. Series D. Animal Science, Vol. LVIII
Written by Khusnul KHOTIMAH, Karina Restu FITRIANA, Ratu SAFITRI, Mia MIRANTI, Lobo Balia ROOSTITA, Hartati CHAIRUNNISA
Research about characterization of probiotic Lactobacillus sp. origin from bovine colostrum in some types of formula encapsulation has been done. The research method was carried out experimentally using a factorial Completly Randomized Design with two factor: a x b, the value of a and b depend on the level of each treatment, which a was bacteria that encapsulated in some types of formula and b was characterization of probiotic test, with three times repetition. The data were analyzed using analysis of variance with a level of 95%, followed by Duncan's Multiple Range Test. The results showed that L. paracasei in alginate tapioca and L. curvatus in alginate skim had high viability during storage of 4 weeks with each of the bacterial population were 2,98 x 1010 CFU/ml and 2,25 x 1010 CFU/ml. Bacteria L. curvatus encapsulated in alginate skim had the highest resistant to the acidic environment of pH 2 and 4 with each of the bacterial population were 1,02 x 103 CFU/ml and 3,69 x 105 CFU/ml and resistant to bile salt concentration of 0.3% and 0 , 5% with each of the bacterial population were 3,5 x 1010 CFU/ml and 2,4 x 1010 CFU/ml. The supernatant of L. curvatus in alginate skim also had the highest antimicrobial activity against pathogenic bacteria Escherichia coli and Salmonella typhimurium with each inhibitory diameter was 20.83 mm and 12.67 mm. Encapsulation formula, especially the combination of alginate and skim milk, can protect the probiotic during processed, storage, and applied in digestive tract.
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