SPIN-SPIN PROTON TRANSVERSE RELAXATION TIMES STUDIES OF RED BLOOD CELL MEMBRANE IN RABBITS WITH EXPERIMENTAL ATHEROSCLEROSIS

Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Cristian Romeo REVNIC, Flory REVNIC, Cătălina PENA, Bogdan PĂLTINEANU, Silviu VOINEA

Nuclear magnetic resonance (NMR) is a modern and accessible technique for studies of erythrocyte membrane permeability in physiological and pathological conditions. In this paper we investigated in rabbits fed on high reach cholesterol diet, by nuclear magnetic resonance method (NMR) the following parameters: the proton life time in erythrocyte, in erythrocyte sediment proton transverse relaxation times (T2a), proton transverse relaxation times in plasma (T2b) and erythrocyte membrane permeability (EMPW). Investigations were carried out on 12 male rabbits aged 20 months old divided into two groups of 6 rabbits each: group A controls and group B fed on cholesterol reach diet (animal origin) for 8 weeks. 1H NMR measurements of the above parameters were performed with an Aremi'78 Spectrometer at 25 mHz frequency. There was a decrease in proton transverse relaxation times in red blood cells from rabbits fed on cholesterol reach diet which suggests an accelerated proton exchange. The activation energy of water exchange through erythrocyte membrane (E) is decreased in cholesterol fed rabbits versus controls. This means that at higher levels of cholesterol the exchanges of water become more accelerated and the processes of membrane exchange are partially disconnected under the influence of thermal processes with heat liberation. In other words, in controls the water exchange processes through erythrocyte membranes increases in parallel with the increase in local or global temperature due to metabolic reactions with heat liberation in intracellular environment. Erythrocyte membrane permeability to water can be taken into consideration as an index of cardiovascular system recovery, important in maintaining a dynamic equilibrium with vascular destruction phenomenon due to high blood pressure.

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REVNIC C.R., REVNIC F., PENA C., PĂLTINEANU B., VOINEA S. 2017, SPIN-SPIN PROTON TRANSVERSE RELAXATION TIMES STUDIES OF RED BLOOD CELL MEMBRANE IN RABBITS WITH EXPERIMENTAL ATHEROSCLEROSIS. Scientific Papers. Series D. Animal Science, Vol. LX, ISSN 2285-5750, 174-182.


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