DETERMINATION OF RAT ADULTERATION IN MEATBALLS USING ENZYME - LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUES AT JATINANGOR EDUCATION AREA, SUMEDANG DISTRICT, WEST JAVA, INDONESIA

Published in Scientific Papers. Series D. Animal Science, Vol. LXI, Issue 1
Written by Roostita Lobo BALIA, Gemilang Lara UTAMA, Eka WULANDARI, Lilis SURYANINGSIH, Andry PRATAMA

Currently, one of the most significant threats is the excessive hunting on wild animals. This is because the hunting results into a food product with turnover and big profit. The trigger for the demand for animal protein derived from the flesh of wild animals (bush meat) against certain species may lead to an increase in diseases. Adulteration processed meatballs into one type of processed replaces raw materials with rat meat. In addition, to causing economic losses, the food products consumed are not safe, healthy, whole, and halal, therefore the need for identification of these food products appears. One method that can be used to detect food product adulteration is the ELISA (Enzyme-Linked Immunosorbent Assay). This test method is an effort to detect the presence of antibodies or specific antigen in a sample. A total of 29 samples were collected from various meatball traders and chicken noodles around Jatinangor. The results showed negative results of 96.55% and positive results 3.45%. A positive result has a greater value than a negative result color (blue) indicating that rat antibody samples bind to streptavidin-peroxidase antigens, where antigen and antibodies occur in homologous process resulting in a change of color (yellow). This test has a high sensitivity level, so that on products that have experienced ripening can still be detected specific antigen. This is evidenced in the positive control of rat meatballs, and antibodies in rat can still be detected. The negative results indicate there is no adulteration meat meatball in the meatball and it is an evidence that Halal in the area of Jatinangor and surrounding areas become an important concern on food in the campus environment.

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BALIA R.L., UTAMA G.L., WULANDARI E., SURYANINGSIH L., PRATAMA A. 2018, DETERMINATION OF RAT ADULTERATION IN MEATBALLS USING ENZYME - LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUES AT JATINANGOR EDUCATION AREA, SUMEDANG DISTRICT, WEST JAVA, INDONESIA. Scientific Papers. Series D. Animal Science, Vol. LXI, Issue 1, ISSN 2285-5750, 299-302.


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