Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 2
Written by Mirela CREȚU, Victor CRISTEA, Angelica DOCAN, Lorena DEDIU, Raluca - Cristina ANDREI (GURIENCU), Anca-Nicoleta CORDELI (SĂVESCU)
The aim of this study was to evaluate the haematological profile of rainbow trout after applying of different intensities of feeding (FL1-2.5% BW day-1, FL2-3% BW day-1, FL3-3.5% BW day-1, FL4-4 % BW day-1, FL5-4.5 % BW day-1 and, ad libitum-FL6). Fish were reared in a recirculating aquaculture system (RAS), provided with twelve rearing units (in duplicate) for 44 days. Blood samples were collected from twelve fish on each experimental variant, and were made before and after the experimental trial. Red blood cell counts (RBCc), haematocrit values (Hct), haemoglobin concentration (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total serum glucose (Glu) and total proteins (TP) were determined. Also, in order to determine the leukogram and the absolute number of leukocytes, blood samples were collected and used immediately to make smears. At the end of the experiment, ANOVA test revealed no significant changes (p>0.05) in the RBCc, Hct, Hb, MCV, MCH, MCHC and glucose concentration (Glu), while the total proteins (TP) from blood serum increased significantly (p˂0.05) with the increasing of the feeding intensity, except for ad libitum feeding, were total serum protein decreased. Regarding the total number of leukocytes, in general, it has not been registered statistically significant changes (p˃0.05). Lymphocytes (relative and absolute) have remained relatively constant, without significant variation (p˃0.05). The main conclusion that emerges from this current study shows that in the case of rainbow trout reared under recirculating aquaculture system conditions, feeding intensity does not significantly influence the haematological profile.
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