Published in Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 1
Written by Madalina Alexandra DAVIDESCU, Daniel SIMEANU, Cristina SIMEANU, Mihaela IVANCIA, Steofil CREANGA
This research aims to validate the most effective method of extracting DNA from a number of 20 blood samples collected from cows. Two methods were tested, namely: DNA extraction using a manual extraction kit-Promega and automatic DNA extraction, using the Maxwell 16 LEV Blood DNA kit-Promega. Following the quantification of DNA samples, by spectrophotometry technique, the best results were obtained by applying the automatic extraction method (77.37 ng/µl DNA concentration obtained by automatic extraction compared to 14.95 ng/µl DNA concentration obtained by manual extraction). Therefore, the effectiveness of this technique has been demonstrated, representing a first step in genomic analysis protocols. The accuracy of the subsequent results depends to a large extent on the results obtained by extracting the DNA from the samples. Therefore, the automatic DNA isolation method is recommended because it has a number of advantages: accuracy, reduced analysis time, low costs, reduced labor and ease of application. This technique can be successfully applied in the analysis of genetic diversity of different animal species.
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{simplepopup popup="false" name="Citation4"}DAVIDESCU M.A., SIMEANU D., SIMEANU C., IVANCIA M., CREANGA S. 2022, TESTING THE EFFECTIVENESS OF TWO METHODS OF EXTRACTING DNA FROM BLOOD SAMPLES FROM COWS. Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 1, ISSN 2285-5750, 40-45. {/simplepopup}