Published in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Vladimir BUZAN
Lipids are integral and essential components of the membranes that perform multiple functions in the life of germ cells. They provide the energy reserve, the solubility of various vitamins, the physical thermoregulation of activity of membrane-bound enzymes and other. In accordance with modern data about the molecular organization of biological membranes, the lipids are represented mainly by amphiphile connections: phospholipids, glycolipids and cholesterol. At the same time phospholipids make up more than 60% of the total number of membrane lipids. In combination with cholesterol they provide fluidity and plastic properties of biological membranes. Based on the above, the purpose of the conducted research was to study the content of phospholipids, their fractions and to determine the possibility of their regulation during hypothermic storage of boar semen at 16-18°C. Using the method of thin-layer chromatography allowed allocating and identifying 6 phospholipid fractions in the reproductive cells of the boar. Among them prevail phosphatidylcholine and phosphatidylethanolamine, whereas phosphatidylserine and cardiolipin presents minor factions. In the process of dilution of sperm is observed multidirectional change in their content. It is established that targeted stabilization of the content of phospholipid fractions at the level of native spermatozoa is possible by improving of synthetic mediums. The obtained experimental data can be used for solving the problems of reproduction of pigs.
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