Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Mangesh NAKADE, Krisztián BALOGH, Laura FOGARASI, Judit SZABÓ-FODOR, Erika ZÁNDOKI, Miklós MÉZES, Márta ERDÉLYI
The purpose of present study was to investigate the short-term effect of DON and T-2 toxin exposure on some blood and liver lipid peroxide and glutathione redox parameters in broiler chickens. A total of 120 three-week old Cobb 540 broiler chickens were randomly assigned into five experimental groups of 24 chickens in each. The short-term trial lasted for 48 hours, after 12 hours of feed deprivation. The experimentally mycotoxin-contaminated diets contained (1 kg) 3.74 mg T-2 or 16.12 mg DON, respectively. Herbal mixture (Herbamix Basic Premix™, Herbamix Trade Ltd., Budapest) was added to the complete feed at the dose of 600 mg/kg. Six birds of each group were slaughtered at 12th, 24th, 36th and 48th hours of the experiment. Parameters of the lipid peroxidation (malondialdehyde) and the glutathione redox system (reduced glutathione content and glutathione peroxidase activity) were measured in blood plasma and liver homogenate. Malondialdehyde content did not change in blood plasma but it was significantly lower in liver homogenate in both mycotoxin loaded groups fed with herbal mixture supplemented feed at 24 hour as compared to the control. Reduced glutathione content did not change significantly in blood plasma, but in liver homogenate, at 24 hour sampling, T-2 toxin alone or in combination with herbal mixture showed significantly higher values as compared to the control. In conclusion, the investigated trichothecene mycotoxins at the dose applied, activated the glutathione redox system in liver of broiler chicken, while addition of herbal mixture has moderate effect against the mild oxidative stress as caused by DON or T-2 toxin.
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