Published in Scientific Papers. Series D. Animal Science, Vol. LXI, Issue 1
Written by Ksenia KULIKOVA, Yusupjan YULDASHBAEV, Salaudi HATATAEV
The Tuvan short-fat-tailed sheep is a local breed spread in the Russian Federation. This breed habites in Tuva. The aim of investigation was the identification of the genetic polymorphism of calpastatin (CAST) and the growth differentiation factor-9 (GDF9) genes in the Tuvan local sheep population. Calpastatin gene is known as a candidate gene of meat quality traits, and GDF9 gene is a potential genetic marker of prolificacy. Genomic DNA was isolated from samples of blood of 131 animals. Two primer pairs were used to obtain 622 b.p. fragment of CAST gene and 462 b.p. fragment of GDF9 gene. Calpastatin locus was digested with MspI restriction enzyme. Two genotypes (MM and MN) of CAST gene were observed. The polymorphism of GDF9 gene (CC and CD genotypes) was detected after amplicons digestion with AspLEI restriction enzyme. MM and MN genotypes were identified with 0.855 and 0.145 frequencies, M and N allele frequencies were 0.928 and 0.072, respectively. In this population CC and CD genotypes of GDF9 gene were identified with 0.878 and 0.122 frequencies, C and D allele frequencies were 0.939 and 0.061, respectively.
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