Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Flory REVNIC, Cristian Romeo REVNIC, Silviu VOINEA
Our study done on experimental hypertensive old rats model using reach cholesterol diet associated or not with rejuvenating drugs (Aslavital/procaine was designed to investigate biophysical parameters such as the proton transverse relaxing times of intracellular free and bound water in liver tissue and of membrane permeability to water by 1H NMR method..Biological material: 32 male white Wistar rats aged 24 months old were divided into 4 groups of 8 rats each. Group A (control) group (B) received only reach cholesterol diet for 6 weeks without associated eutrophic substances. Group (C) which received high cholesterol diet associated with Aslavital treatment for 6 weeks 4mg/kg body weight intraperitoneal injections(ip). Group (D) has received associated treatment with Procain 4mg/kg body weight in order to test the antiatherosclerotic properties of this rejuvenation drug. In liver tissue, bound water , characerised by structural parameter T21 presents a slight decrease în treated groups, comparatively with Control group, while free water characterised by structural parameter T22, decreases following cholesterol administration and increases under Aslavital and Procaine treatment, in the case of Procaine this effect is more powerfull above the control values. In the case of intraerythrocyte water there are very subtile changes , but which remind of those which take place in the liver. As a mirrror reflexion of the behaviour previosly described , the proton trannsverse relaxing time of plasma water increases slightly in cholesterol treated rats and decreases under the actions of administrated drugs ,with a more pronounced effect under Procaine treatment. Conclusion: Liver hypertophy is associated with a decrease in proton transverse relaxing times as well as in the proportion of free water(m2%), associated with the decrease in proton transverse relaxing times as a consequence of the increase in protein content of liver tissue.The global decrease of proton transverse relaxation time (T2) in liver is mainly due to changes in the free and bound water ratio and of a decrease in proton transverse relaxing times of free water(T22) and bound water(T21). The effects of Procaine are much more increased. In general, Aslavital is behaving like a buffered Procaine with a more adequate action. There were cases in which both drugs had the same effects:for example a decrease in proton transverse relaxing time T22, characteristic of bound water structural parameter, or did not manifest any influence upon proton transverse relaxation time T21 charateristic for free water structural parameter from liver.
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