Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Enkeleda BERBERI, Ariola DEVOLLI, Ilir LLOHA, Kevin MICI
Aeromonas salmonicida is a bacterial pathogen that causes infection mainly in Salmonidae family. The objective of this ongoing study was to isolate and identify the bacterial pathogen in Lake Ohrid, since the main fish species that populate this aquatic body belongs to the Salmonidae family. Salmo letnica and Salmo ohridanus are the main species where we have focused our analyses. These fish species not only are one of the main catches but also one of the main foods in the region around Lake Ohrid. Sampling was carried out during spring and autumn season in 2017. Material from liver and skin mucus from each sample was used for the inoculation of the TSA medium. The incubation period was between 5-7 days at 180C which was followed biochemical test. PCR was performed by using MIY primer (MIY1 5’-AGCCTCCACGCGCTCACAGC-3’ and MIY2 5’-AAGAGGCCCCATAGTGTGGG-3’). All the data were analyzed with Sigma Plot 12.5. A multiple Comparison Procedures (Tukey Test) between groups, water temperature, liver bacterial colonies and skin colonies (p<0.05) was performed.
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