Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Madalina Alexandra DAVIDESCU, Mitica CIORPAC, Steofil CREANGA
In molecular genetics analysis, isolation and quantification of DNA represents a step extremely important. DNA isolation methods are based on purity, integrity and the amount of DNA obtained. The degree of DNA purity is one of the most important factors in the reproducibility of the PCR method. DNA is considered pure if the ratio of the two spectrophotometric readings, A260/A280, shows values between 1.7 - 2.0. Values lower than 1.7 indicate impurities with proteins, and a ratio higher than 2.0 indicates impurities with other contaminants. The aim of this research was to evaluate the effectiveness of the automatic method of DNA extraction from a number of 24 blood samples collected from Pinzgauer cattle breed, for the analysis of genetic diversity. The amounts of DNA extracted from the 24 blood samples ranged from 13-61.9 ng/μl and the absorbance ratio A260/A280 showed values between 1.61-2.13. The results obtained demonstrated the effectiveness of the automatic method of DNA extraction, and thus, isolated and quantified genetic material can be used further in the next stages of genomic analysis of this breed.
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