Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Ion BALAN, Gheorghe BORONCIUC, Vladimir BUZAN, Nicolae ROŞCA, Ion MEREUŢA, Iulia CAZACOV, Alexandru DUBALARI, Irina BLÎNDU, Nicolai FIODOROV
Increasing the efficiency of reproduction of farm animals is possible through the use of progressive cryotechnology. Numerous studies have been devoted to this problem. However, a significant number of cells do not restore their functional activity after deconservation, which hinders a fuller use of the genetic potential of high-value breeding animals. Therefore, the purpose of the research was to determine the optimal technological parameters of cryopreservation. To achieve the intended goal, experiments were carried out to determine: the optimal rate of cooled semen up to 4°C, the temperature of the fluoroplastic plate, the duration of cooling of bull sperm after diluting it with mediums containing various cryoprotective agents. It has been established that when developing new mediums, it is necessary to use the optimal modes of cryopreservation of sperm of different types of farm animals. The results presented in this paper allow us to understand that cryotechnologies should be developed taking into account the features of the frozen object and the physicochemical properties of the components of protective mediums.
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