Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Laura M. DALE, André THEWIS, Ioan ROTAR, Christelle BOUDRY, Roxana VIDICAN, Anamaria MALINAS, Vasile FLORIAN, Bernard LECLER, Richard AGNEESSENS, Juan A. FERNÁNDEZ PIERNA, Vincent BAETEN

Alfalfa (Medicago sativa L.) is a high quality forage which has been used worldwide. The superiority of alfalfa lies in its high yield, high protein content and high digestibility. The aim of this study was to develop a simple, fast and non­destructive method, named Near Infrared Spectroscopy (NIRS) to determinate alfalfa quality. To realize this study, alfalfa samples were obtained from Manaútur Experimental Station – Farm Cojocna in 2008–2009, in one experiment carried out using randomization blocks design with two experimental factors (mineral fertilization and period of harvest). Alfalfa quality was first determinated on 48 samples by classical analyses: crude protein CP (AOAC, 1990), crude fiber CF (Fiber Cap, FOSS, DK) and in vitro organic matter digestibility OMDrt (DeBoever, 1986). Then the samples were scanned by NIRS. Calibration models were performed by PerkinElmer Spectrum Quant + 4.21 program (USA) on the 48 samples determinated by classical analysis. The results showed fully confirmed by acceptable coefficients of determination and standard error of cross-validation (R2=0.96 for CP, 0.94 for CF, 0.98 for OMDrt and SECV=0.77 for CP, 1.35 for CF, 1.13 for OMDrt). Successful results for prediction of other 176 alfalfa samples were then obtained using these calibration models: SEP=0.869 for CP, 1.058 for CF, 1.058 for OMDrt). The highest CP and OMDrt values of alfalfa were obtained in bud stage (22.0% and 66.0% respectively). While for CF, the highest content was registered in the seed formation stage (46.0%). The NIRS technique offers us the possibility to determinate rapidly and easily Romanian alfalfa important parameters, but the system could also be used for the determination of other constituents.

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{tag}meta name="citation_title" content="Determination of romanian alfalfa crude protein and crude fiber contents as well as in vitro organic matter digestibility by nir spectrometry"{/tag} {tag}meta name="citation_journal_title" content="Scientific Papers. Series D. Animal Science"{/tag} {tag}meta name="citation_publication_date" content="2012"{/tag} {tag}meta name="citation_author" content="Laura M. DALE" {/tag} {tag}meta name="citation_author" content=" André THEWIS" {/tag} {tag}meta name="citation_author" content=" Ioan ROTAR" {/tag} {tag}meta name="citation_author" content=" Christelle BOUDRY" {/tag} {tag}meta name="citation_author" content=" Roxana VIDICAN" {/tag} {tag}meta name="citation_author" content=" Anamaria MALINAS" {/tag} {tag}meta name="citation_author" content=" Vasile FLORIAN" {/tag} {tag}meta name="citation_author" content=" Bernard LECLER" {/tag} {tag}meta name="citation_author" content=" Richard AGNEESSENS" {/tag} {tag}meta name="citation_author" content=" Juan A. FERNÁNDEZ PIERNA" {/tag} {tag}meta name="citation_author" content=" Vincent BAETEN " {/tag} 

alfalfa

crude fiber

crude protein

in vitro organic matter digestibility

NIRS