Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by Bogdan Paltineanu, Cosmin Sonea, Cătălina Pena, Flory Revnic, Cristian Romeo Revnic
Ln order to obtain new data concerning muscle contraction at the molecular level, the interrelation water-contractile proteins has been investigated by means of 1 H NMR Spectroscopy. Rat heart muscle from 6 and 37 months old rats has been used for proton transverse relaxation time measurements in Ri, Co and Re. at different [ATP].The distribution of negative charges in contraction and relaxation has been measured by exposing glycerinated muscle from 6 to 37 months old rats to different [Mn+2].Our data have pointed out the existence of two proton relaxation tinies:T2s and 121 accounted for two water compartments. The modification in water state are related with modifications in contractile activity. The elongation od proton transverse relaxation times is associated with a decrease in the degree of water molecules aggregation.T2s and T2l are correlated with a reduction in muscle hydration, contraction being a function of ions binding to the protein sites. These sites are implicated in determination of protein hydration state.
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{tag}meta name="citation_title" content="The management of water state in glycerinated rat heart the role of 1H NMR spectroscopy"{/tag} {tag}meta name="citation_journal_title" content="Scientific Papers. Series D. Animal Science"{/tag} {tag}meta name="citation_publication_date" content="2012"{/tag} {tag}meta name="citation_author" content="Bogdan Paltineanu" {/tag} {tag}meta name="citation_author" content=" Cosmin Sonea" {/tag} {tag}meta name="citation_author" content=" Cătălina Pena" {/tag} {tag}meta name="citation_author" content=" Flory Revnic" {/tag} {tag}meta name="citation_author" content=" Cristian Romeo Revnic" {/tag}
1 H NMR
