Published in Scientific Papers. Series D. Animal Science., Vol. LVI
Written by Nezih ATA, Ibrahim CEMAL
This study was carried out to determine Calpastatin gene polymorphism in native Çine Çapari and synthetic Karya sheep in Turkey. Calpastatin is an endogenous inhibitor of calpain. This gene has a key role on meat tenderness after slaughter, and also has been known as candidate gene in muscle growth efficiency. Calpastatin gene was located on 5th chromosome of sheep. Randomly taken blood samples were collected from 97 Çine Çapari and 90 Karya sheep raised in Western Anatolia. Intron I from L domain of the ovine calpastatin gene was amplified by PCR to produce a 565 bp fragment. Then, PCR products were digested with restriction endonuclease enzyme MspI. Digested products were separated by electrophoresis on agarose gel and visualized with gel documentation system. The digestion of the PCR products by MspI enzyme produced fragments of 306 and 259 bp. Data analysis was done using PopGen32 software. In Karya sheep population MM, MN and NN genotypes were identified with 0.296, 0.496 and 0.208 frequencies, M and N allele frequencies were identified with 0.544 and 0.456, respectively. In Çine Çapari sheep population MM, MN and NN genotypes were identified with 0.543, 0,388 and 0.069 frequencies, M and N allele frequencies were identified with 0.737 and 0.26, respectively.
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Calpastatin
