Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Dawar Hameed MUGHAL, Ijaz AHAMD, Muhammad Shehbaz YOUSAF, Umer FAROOQ, Fazal WADOOD, Amjad RIAZ
The present study was designed to: 1) study the influence of various concentrations of trehalose on buffalo bull spermatozoa extended in lactose- egg yolk-glycerol extender (LEYGE) and 2) compare the fertility rate between LEYGE supplemented with an optimal level of trehalose and traditionally used tris-citric-acid-fructose extender. Semen of Nili- Ravi buffalo bulls (n = 04) routinely used for artificial insemination, was collected using an AV at SPU once a week, for eight weeks. Semen samples with ≥70 % spermatozoa motility were pooled and diluted at 37°C in LEYGE extender containing trehalose at 0.0, 30.0, 50.0 and 70.0 mM. The sperm motility, viability, acrosomal integrity, plasma membrane integrity, and DNA integrity were significantly (P≤0.05) higher in LEYGE supplemented with 70.0 mM trehalose as compared to other groups. The fertility rate was significantly (P≤0.05) higher when semen doses extended in LEYGE and supplemented with 70.0 mM trehalose were used (n = 19; 38%) as compared to semen doses extended in the conventional tris-citric-acid-fructose extender (n = 27; 54%). In conclusion, 70 mM is an optimal inclusion level in buffalo bull semen for trehalose in LEYGE in respect to seminal attributes. Furthermore, LEYGE supplemented with 70 mM trehalose presented higher fertility rate in buffaloes as compared to traditionally used tris-citric-acid-fructose extender under field conditions.
[Read full article] [Citation]
