Published in Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 1
Written by Ivona DIMITROVA, Milena BOZHILOVA-SAKOVA, Neviana STANCHEVA
This experiment was conducted in order to be identified the allelic and genotypic polymorphisms of FABP3 (heart-type fatty acid binding protein) gene in 30 ewes from Bulgarian Dairy Synthetic Population breed reared in Experimental base - Tzarev Brod - part of the Agricultural Institute - Shumen. FABP3 gene is a candidate marker that influences milk fat content and marbling of meat. Thirty blood samples were collected from v. jugularis in vacuum tubes with EDTA. Genomic DNA was extracted manually with commercial kit. By means of PCR-RFLP technique with endonuclease BseDI in exon 2 of FABP3 gene (SNP3) were determined the allele and genotype variants of the investigated animals. In this population were observed two alleles - wild allele A with frequency 0.15 and mutant allele G - with 0.85. Two different genotypes were identified - homozygous GG with frequency 0.67 and heterozygous genotype AG with frequency 0.33. Ho (observed heterozygosity) was 0.330 and He (expected heterozygosity) was 0.055. This herd was found to be in Hardy-Weinberg equilibrium (p>0.05).
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