Published in Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 1
Written by Mugurel COLA, Florica COLA
The concept of prudent usage of the antibiotics supposes that their application should have the greatest effect on human and animal health and they should determine the weakest bacteria resistance to the antibiotic used. Among the 48 milk samples assessed, 4 samples (8,33%) were positive according to the test accomplished with the Ecotest device. After 10 minutes of incubation, 91,67% of the samples had enough lactic acid. The lactic acid determined acid pH and phenolphthalein in acid environment is colourless. The test tubes containing the milk from these samples stayed white (the colour of the milk). For the rest of the milk samples (8,33%), because of the presence of antibiotic waste, the active (microbiological) substance did not develop and, since there was no lactic acid, the pH in these test tubes is slightly alkaline or neutral and the phenolphthalein becomes pink. The device used is responsive enough to find the β-lactam antibiotics in milk and it may be used at the farm level. The antibiotic concentration according to the “screening” was under the maximum admitted limit (4 µg/l) and all of the 4 samples were “screen positive”.
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