Published in Scientific Papers. Series D. Animal Science, Vol. LXV, Issue 2
Written by Yosyp RIVIS, Daniel ZABORSKI, Bogdan GUTYJ, Olga HOPANENKO, Оleksandr DIACHENKO, Olga STADNYTSKA, Oleg KLUM, Ivan SARANCHUK, Vasyl BRATYUK, Vasyl FEDAK
The literature describes non-quantitative gas chromatographic methods for determining the relative content of various forms of long chain fatty acids in the biological material under study. Our task was to improve the quantitative and simultaneous gas chromatographic method for determining the absolute content of various forms of long-chain fatty acids in the biological material under study (plant and animal tissues and liquids). To do this, the studied biological material, a simple and complex internal standard is treated with various extracting mixtures. Various forms of long chain fatty acids are isolated from extracted lipids and their methyl esters are chromatographed. The results of gas chromatographic studies are calibrated. The improved method for low error allows quantitatively and simultaneously in absolute units to determine the content of total long chain fatty acids, long chain fatty acids of common lipids, esterified, non-esterified and anionic long chain fatty acids in the studied biological material. Thus, using the improved gas-chromatographic method at a lower cost of reagents and time, quantitatively and simultaneously in the biological material under study in absolute units we determine the content of various forms of long chain fatty acids.
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