Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Valeria Cristina BULGARU, Gina Cecilia PISTOL, Ionelia ȚĂRANU, Daniela Eliza MARIN
Feed contaminants, especially mycotoxins are responsible for important economic loses in swine industry, pigs being very susceptible to contamination with mycotoxins. Alternariol (AOH) is a mycotoxin synthesised by fungi of genus Alternaria and is a common contaminant of different raw materials, as cereal and cereal products that represent important ingredients of swine diets. Recent literature data have shown that in human cell model, AOH can cause DNA damage and induce oxidative stress. However, the effect of AOH in swine it less documented. Consequently, the aim of our research was to investigate how AOH can affect the proliferation, apoptosis, death, and cell cycle using an in vitro model represented by PBMCs (peripheral blood mononuclear cells) isolated from pig's blood. Cells were stimulated or not with phorbol 12-myristate13-acetate-ionomycin (PMAI), exposed for 4h to different concentrations of AOH (1-100 µg/mL). Our study shows that AOH was able to affect cell proliferation, apoptosis, cell death and cell cycle of porcine PBMCs, with negative effects on the animal health.
[Read full article] [Citation]
