Published in Scientific Papers. Series D. Animal Science, Vol. LV
Written by El-Sharawy, M. E.; El-Shamaa, I. S.; Ibrahim, M.A.R.; Abd El-Razek, I.M.; El-Seify, E.M.
Semen from three Egyptian buffalo bulls was collected once weekly and ejaculates with more 75% progressive motility and more 85 % normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bulls effect. Seven extenders were used: Tris 20 % egg yolk extender with 7 ml glycerol as a control (T1), and substitution of whole egg yolk with 4, 6, 8, 10, 12 and 15 % low density lipoprotein (LDL), T2 – T6, respectively. Semen was diluted to 80 x106 sperm/ml, packaged into 0.25 ml straws, cooled, held at 5.C for 4 h, and then frozen in liquid nitrogen (LN) and stored at -196.C for at least one month. Sperm progressive motility, intact acrosome and plasma membrane integrity were assesd at post dilution, equilibration, post-thawing (at 37.C for 30 sec.) and after 30 days storage in LN. This study reveled that LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buffalo spermatozoa than whole egg yolk extender. Sperm progressive, intact acrosome and plasma membrane integrity were much higher (P < 0.05) in the 12% LDL extender (63.3, 77.17 and 71.3% respectively) vs. 35, 40.8 and 34.7% in the control 20% EY extender at post-thawing process, respectively. Fertility rates were higher in extender containing 12% LDLs compared with the control (72.7% vs. 50%, respectively). It was concluded that LDL (12%) in extender improved the freezability and fertility of buffalo bull spermatozoa.
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{tag}meta name="citation_title" content="Effect of low density lipoproteins in extender on freezability and fertility of egyptian buffalo bull semen"{/tag} {tag}meta name="citation_journal_title" content="Scientific Papers. Series D. Animal Science"{/tag} {tag}meta name="citation_publication_date" content="2012"{/tag} {tag}meta name="citation_author" content="El-Sharawy" {/tag} {tag}meta name="citation_author" content=" M. E.; El-Shamaa" {/tag} {tag}meta name="citation_author" content=" I. S.; Ibrahim" {/tag} {tag}meta name="citation_author" content=" M.A.R.; Abd El-Razek" {/tag} {tag}meta name="citation_author" content=" I.M.; El-Seify" {/tag} {tag}meta name="citation_author" content=" E.M." {/tag}
Buffalo bull semen
