Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Maryam CHAUDHARY, Umer FAROOQ, Musadiq IDRIS, Mushtaq Hussain LASHARI, Haroon RASHID, Maheen ANJUM, Asad ALI, Abrar AFZAL, Nasrullah KHAN
The present study was conducted with objective to evaluate the validity of three hemoglobin (Hb) estimation methods, including the cyanmethemoglobin method (HbC), Sahli's method (HbS), and veterinary hematology analyzer (HbA) in Cholistani cattle (n=100). Blood samples were collected aseptically from apparently healthy cattle. The results regarding the overall data and the data for age, and sex-wise groups revealed that HbA was significantly (p ≤ 0.05) different from HbC, whereas HbS was non-significantly (p ≤ 0.05) different from the HbC. The Bland and Altman chart between HbS and HbC, and between HbA and HbC showed significantly higher level of agreement between HbS and HbC with no proportional bias on the distribution of data around the mean difference line (Mean= 0.39, 95% CI= 0.21 to 0.57). Cronbach’s alpha and intraclass correlation coefficient between HbS and HbC, and between HbA and HbC for single and average values, on similar grounds, were also higher between HbS and HbC being 0.819 and 0.900 as compared to the values of 0.793, and 0.884 between HbA and HbC. Sahli's method (a three-time average) for Hb estimation in cattle blood is comparable to the gold standard technique of the cyanmethemoglobin method, endorsing its use as a point-of-care testing device in remote areas.
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