Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Vasile MACIUC, Ion NECHIFOR, Alexandru Marian FLOREA, Bogdan Ioan NECHIFOR, Ioana ȚURCANU, Ana BOLDIȘOR, Daniel Constantin NECHIFOR, Constantin PASCAL
The present work aimed to genotyping sheep in order to know the predisposition to scrapie and take the necessary measures. The analyzed biological material was represented by 822 pure breed Karakul sheep and by 239 crossbred meat sheep obtained by crosses between females of the Karakul breed and males of the Palas meat line. The method of determining the genotypes of susceptible sheep to scrapie from biological samples, consists in the analysis of the coding region of the PRNP gene (exon 3) where there are three codons associated with the resistance to this disease. Purebred Karakul sheep which have the genotypes belonging to classes 1 and 2, with the highest resistance to scrapie, have the share of 25.70%, compared to crossbred meat sheep in which the share of genotypes for the mentioned classes was 58.70% (significant for p<0.01 and C.I = 95%). An increase in resistance to scrapie was found in crossbred meat sheep which proves that by practicing infusion crosses with disease-resistant breeds, we will be able to increase scrapie resistance in the case of purebred Karakul sheep.
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