Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Oleg CHISELIȚA, Grigore DARIE, Doina ROTARI, Natalia CHISELIȚA, Alina BEȘLIU, Nadejda EFREMOVA, Mariana CARAMAN
The purpose of the work was to evaluate the influence of a mannoprotein extract (MP) from brewer yeast on the motility, speed, as well as the morphological and microbiological indices of the ram semen preserved by refrigeration at +4°C. The experimental extender (EE) supplemented with MP at the concentrations of 0.6-0.9% v/v significantly increased (P≤0.05) the total motility (TM) and the progressive motility (PM) of ram spermatozoa during 96 hours of storage and did not negatively influence the VAP, VSL and VCL indices. At the end of the 96-120-hour period of storage MP in the concentrations of 0.8-1.0% v/v contributed to reduction of the abnormal sperm (AS) number as compared to the control extender (CE). EE with MP in the concentrations of 0.2-1.0% v/v significantly reduced (P≤0.05) the contamination of the semen samples with various microorganisms. The obtained results demonstrated perspectivity of the yeast extracts for effective semen dilution and preservation for further artificial insemination (AI) of animals.
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