Published in Scientific Papers. Series D. Animal Science, Vol. LXI, Issue 1
Written by Vladimir BUZAN, Gheorghe BORONCIUC, Ion BĂLAN, Iulia CAZACOV, Nicolae ROŞCA, Melania BUCARCIUC, Ion MEREUŢĂ, Alexandru DUBALARI
By the methods of spectrophotometric, chromatographic and morphological studies established that as a result of influence of the temperature factor takes place adaptation-compensatory reactions in the gamete plasma membranes of farm animals. As a result of the adaptive-compensatory reactions in the plasma membranes of bull and boar gametes to the influence of temperature factor occur a shift of the phase transition of lipids to a lower temperature. In plasma membranes the amount of phospholipids predominates over cholesterol. During the stages of cryopreservation of cock semen there is a decrease in the amount of cerebrosides of 1, 5, 6, and 8 fraction, which compensates by increasing of quantity of cerebrosides third faction. In a similar direction changes the phospholipid-cholesterol ratio, which tends to unity, that is, in the direction of eliminating the phase transitions of lipids. The observed processes may be a consequence of a change in the activity of the phospholipase system, which is a gamete protective reaction in response to the action of extreme cryopreservation factors. The obtained results of the studies of fatty acid composition of plasma membranes testify to its change under the action of cryogenic factors. The accumulated material allows us to note the manifestation of specific reactions characteristic of individual fatty acids during freezing and thawing of biological objects. The change in the content of phospholipids and the saturation of fatty acids of plasma membranes testifies a complex adaptive-compensatory reactions aimed at preserving the viability of gametes in the new conditions of their stay.
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