Published in Scientific Papers. Series D. Animal Science, Vol. LXI, Issue 1
Written by Semra KÜÇÜK
This experiment was devised to evaluate the effects of tricaine methanesulfonate (MS-222) on blue tilapia, Oreochromis aureus at five different salinities (0, 8, 16, 20, 24 ppt) and four different tricaine concentrations (200, 300, 400, 500 mg l-1). Even though, a body of literature exist about the tricaine usage on fish, not much information is present on tricaine with salt. The requirement time to anaesthetize fish depends on intensity of tricaine concentration and salinity. Induction time of fish decreased as tricaine concentrations increased. When exposed to any of tricaine concentrations, fish entered a deep state of anaesthesia (induction time ranged between 0.19 and 2.54 min). Recovery time was highest at 400-500 mg l-1 of tricaine as salinity increased. Tricaine + salt combination is strongly recommended to use in blue tilapia culture. Ideal tricaine concentration was 200 mg l-1 of tricaine at 8 ppt of salinity to reduce stress in blue tilapia.
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