Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 1
Written by Florica BUȘURICU, Verginica SCHRODER, Doina MARGARITTI, Dorina NADOLU, Andreea Hortanse ANGHEL
Legislation enforce allows the use of food additives that have adverse effects on the human body, such as: asthma, contact hives, allergies, digestive disorders, ADHD, cancer. The toxic effect is particularly enhanced when more than one additive is associated in a food. In this context, we propose to study the effect of benzoate ± sorbate on some food colorants (E129 red allura and E133 bluish blue) from 8 fruit juice samples. Sodium benzoate was dosed by the spectrophotometric method using a standard solution Cs = 0.1 mg/mL. Evaluation of the cytotoxicity of these juice additives was followed using the BLSA (Brine shrimp lethality assay) test. For all analyzed samples, the maximum admissible value for benzoate (200mg/L) is observed to be followed. The fastest cytotoxic effects were recorded in the first 24 hours, but at low concentrations of 50 μL/mL and 100 μL/mL. Processes such as cytoplasmic accumulation of inclusion vesicles, disruption of membrane activity, as well as phenomena associated with cell division and differentiation are identified. In conclusion, although all products contain benzoate levels within acceptable limits, there is a risk of accumulation of larger quantities in the body, by consuming products with this preservative. The combination of these preservatives with dangerous dyes increases the toxic potential, which was also confirmed by the BSLA test. Proper product labeling would inform and guide the consumer / patient to balanced consumption with fewer synthetic additives, with the option of choosing an alternative that does not harm the health.
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