Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Kiki HAETAMI, Yuniar MULYANI, Yeni MULYANI, Abun ABUN, Junianto JUNIANTO
Proteolytic bacteria are bacteria that can hydrolyze proteins into smaller peptides or amino acid units. The existence of extracellular protease-producing bacteria is very important for life because it provides the need for nitrogenous compounds and can be used as a probiotic agent. This study was conducted to determine the proteolytic activity of Bacillus sp. Isolates. From the results of the collection and isolation of indigenous bacteria, the gut of carp. The method used is purposive sampling method, the data is analyzed quantitatively descriptive. The material used is the bacterium Bacillus sp. selected from the digestive tract of omnivore fish. Proteolytic test using clear zone method in skim milk agar (SMA) culture was measured at 12th, 24th, and 48th hours. The results showed that nine isolates of Bacillus sp. selected has proteolytic activity. The diameter of the clear zone (14.33-18.33.mm) Bacillus isolates from the 12th hour showed qualitatively the high proteolytic ability of the protease enzyme produced or also the high number of enzymes produced and released out. The optimum pattern of production time is in line with the general bacterial growth curve pattern. The results showed that at the 48th hour, bacterial isolates CP013984_s Bacillus sp. produce the highest protease activity, which is equal to 46.84 mm. The use of bacillus isolates in making probiotics can increase protein content and reduce crude fiber substrate, with a crude protein composition of 31.23%, extract ether 4.38% and crude fiber 8.64%.
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