Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Angelica DOCAN, Lorena DEDIU, Mirela CREȚU
Silurus glanis are an important romanian aquaculture species raised for food and sport fishing, especially in polyculture tehnology but also in the intensive semi-closed and recirculation sistems. The aim of present study was to obtain a basic knowledge of the leukocyte reaction of European catfish under the action of toxic metabolites (aflatoxin B) secreted by the mold Aspergillus flavus. The sampling of blood from the healthy and affected Silurus glanis exemplars allowed determination of white blood cell count. In order to achieve the purpose of the experiment the blood samples were immediately used to make smears which were colored with May-Gründwald Giemsa panoptic method. By studying different types of leucocytes was determined leukograma and absolute number of leukocytes. Physiological stress induced by toxic metabolites secreted by the mold is reflected in the hematological parameters - white blood cell count (significant decrease, p=<0.05). In the infected fish with aflatoxin the total number of leukocytes has been registered statistically significant changes with lymphopenia, neutrocytosisand eosinophilia. The hematological changes of infected fish led to the affect the immune defense system of the Silurus glanis specie.
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