Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Doina ROTARI, Grigore DARIE, Oleg CHISELITA
The research was performed on ram semen. The biological material was the Moldovan Karakul rams. Ejaculates with a mobility of over 70% and a sperm concentration of over 2 billion / ml were allowed for processing. The basic medium used was SȚJ (sucrose, sodium citrate, egg yolk). An additional component introduced in the basic environment was experienced the biologically active preparation LB / MP obtained from yeast from the brewing by the Institute of Microbiology and Biotechnology of the Academy of Sciences of the Republic of Moldova. After diluting and microscopically testing the sperm using the CEROS computer system, the experimental batches were introduced into the cold room at + 2-4 degrees. Mobility assessment was performed at 24 hours intervals. Sperm mobility after 120 hours of storage by refrigeration was maintained at 68.0 ± 5.8 % in the experimental group where the concentration of the preparation in the base medium was 0.8%, mobility allowed for inoculation. Based on the experimental results obtained, it was proposed to dilute and preserve ram semen by refrigerating the SȚJ medium supplemented with 0.8% biologically active preparation LB/MP.
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