ISSN 2285-5750, ISSN CD-ROM 2285-5769, ISSN-L 2285-5750, ISSN Online: 2393 – 2260
 

Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Adrian RĂDUȚĂ, Oana Diana MIHAI, Simona NICOLAE, Ioana Nicole REU, Gabriel COTOR

The research was carried out on 40 Japanese quails (Coturnix japonica), they were divided into two groups of 20 quails each, control and experimental. Both groups were given the same forage regime, combined quail forage, growth phase. The experimental group additionally received a supplement of vitamin C (L-ascorbic acid) and vitamin E (DL-alpha tocopheryl acetate), both vitamins in a dose of 1000 mg/kg of combined feed. The duration of the experiment was 6 weeks. The investigations performed were: body weight, hemogram and some biochemical indices. These investigations were carried out at 21 days and the end of the experimental period, i.e. 42 days. At 21 days and 42 days, significant differences were observed in the individuals of the experimental group: increases in erythrocyte count, haemoglobin and haematocrit were observed. Biochemical investigations showed increases in total protein, alkaline phosphatase and calcemia. Decreases in AST, ALT, blood glucose and creatinine were observed in the experimental group. The body weight was statistically significantly higher in the group receiving the feed diet with vitamin C and E supplementation.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Mevlüt ATALAY, Uçkun Sait UÇAN

Klebsiella species cause infections occurring in different tissues of various hosts. In terms of bovine health it is a well-known opportunistic pathogen playing a role in the pathogenesis of mastitis. In fact, such bacteria can spread widely in bovine farm environments mostly through dairy facilities and breeding areas causing eventually mastitis. Characterizing aetiological agents thoroughly can assist to understand pathogenesis of the opportunistic infections. In this study, a total of 1206 dairy cows from 6 farms were first screened by California Mastitis Test (CMT). Samples found positive by CMT, samples from clinical mastitic udders and also sıwab samples obtained from both the same animals’ rectal and nasal orifices and their surrounding environments were all cultured aerobically and a complete identification of the isolates was achieved by phenotyping and genotyping. Some bovine Klebsiella strains from the culture bank of the Department were also included as organ isolates in the study. Lastly, antibiotic resistance of the strains was detected. There is no difference between numbers of coliforms from the farms using either robotic milking or classical milking systems (p > 0.05). The highest prevalence of Klebsiella mastitis in the farms examined in this study was 8.75%. It was common to see colistin resistance in the Klebsiella isolates from all farms anyway. The lowest 12% and highest 50% resistances for colistin were seen in rectal and organ originated strains, respectively. Unexpectedly, carbapenem (Imipenem) resistance was detected and was the highest 50% in isolates from environments. The lower occurrence of carbapenem resistance 18.2% was measured in Klebsiella spp. isolated from mastitic milk samples. Carbapenem resistancy was further verified molecularly.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Angelica DOCAN, Lorena DEDIU, Mirela CREȚU, Iulia GRECU, Alina MACOVEIU (DOBRE), Ion VASILEAN

Fructose is a highly lipogenic sugar and excessive fructose intake stimulates endogenous glucose production and lipid synthesis in the liver. Therefore, the present study aims to investigate the amelioration of liver impairment induced by high fructose dietary doses following dietary supplementation with krill oil. 45 exemplars of Carassius auratus calico weighing 150 grams, were randomly distributed into three experimental diets including V1 - Control, fish fed with a normal diet, V2 - fish fed with 1% fructose, V3 - fish fed with 2% fructose, respectively, at the end of 90 days: V1 - Control, fish fed with a normal diet, V2 - fish fed with 1% krill oil, V3 - fish fed with 2% krill oil. The blood tissues were collected to analyze hematological parameters (red blood cell counts (RBCc, x 106/µl), the hematocrit (PCV, %), hemoglobin concentrations (Hb, gl/dl,) HEM, VEM, CHEM and serum biochemistry parameters (GLU, TRIG, ALT and AST). In addition, the organosomatic indices (HIS, VSI) and proximate composition (water, lipid, ash, and protein content) were analyzed. No differences between the control and treated fish from the two experimental diets were observed in hematological parameters. After 90 days fructose-treated fish showed increased AST and ALT levels. Our results showed that daily krill oil supplementation in the Carassius auratus calico prevented fructose-induced hypertriglyceridemia.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Bianca-Maria MADESCU, Roxana LAZAR, Alina Narcisa POSTOLACHE, Madalina Alexandra DAVIDESCU, Marius Mihai CIOBANU, Paul Corneliu BOISTEANU

This work aimed to investigate and evaluate the microbiological control of carcasses from Aubrac cattle, focusing on ensuring quality and food safety in the meat industry. In this research, three microbiological parameters were monitored: contamination with Salmonella, total bacterial count and contamination with Enterobacteriaceae. The sponge sampling method was employed, involving the wiping of a 100 cm2 surface area with a template using sponges soaked in sterile diluted peptone. Sampling was carried out randomly from ten carcasses (males and females), selecting four areas with the highest contamination frequency from each carcass, resulting in a total surface area of 400 mm2. Following the tests, it was seen that the samples did not contain any bacteria from the Salmonella genus or the Enterobacteriaceae family. In terms of the total number of bacteria, the highest microbial load was found in males (8.2 x 102 cfu/cm2) on carcasses 2 and 5. Simultaneously, the lowest microbial load (6.0 x 102 cfu/cm2) was recorded on carcass 4 from females. We can conclude that, based on the results, these values are below the accepted standard limit, indicating wholesome meat.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Valentin FOKSHA, Alexandra KONSTANDOGLO, Vasily KURULYUK, V. TICLENKO, Natalya GELETSKY

The article presents the results on the study of key economically beneficial traits of pedigree French Holstein cows in the herd of Society of limited liability "Holstein." It was found that the milk yield of cows in the first lactation averaged 8119 kg of milk, and in the second lactation, it was 8226 kg of milk, which is by 107 kg more than in the first lactation. Comparative analysis of the milk productivity of locally generated cows and their mothers revealed that the daughters' yields (cows of local generation) during the first lactation period of 305 days exceeded the mothers' yields by 1367 kg of milk. In the second lactation, the average yield of locally generated cows was 8505 kg of milk, which is by1253 kg more than the average in the first lactation. A positive correlation was established for milk yield during the 305-day lactation period between the first and second lactations (+0.201). The heritability coefficient for lactation yield was determined to be (h2 = 0.402).

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Genoveva GEORGIEVA, Tsvetomira BANCHEVA, Svetoslava STOYCHEVA

Romanov sheep breed was distributed in Europe more than 50 years ago. It is attracting more and more interest in Bulgaria because of its high fertility, but data on its milk productivity are scarce. The present study aims to determine the milk yield and physico-chemical composition of milk from Romanov sheep as it is the first of its kind in Bulgaria. 137 milk samples obtained from the 30th to the 120th day of lactation of 43 purebred Romanov ewes were analyzed. The average daily milk yield was 0.510 l, reaching 0.720 l as it gradually decreased during lactation. The milk yield for the studied period was 45.7 ± 3.52 l. The percentage content of milk fat increased with advancing lactation, as lactose showed insignificant changes. The protein indicator increased at the beginning, then it slowly decreased.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Petronela Mihaela ROȘU, Cristian Romeo BELU, Dana TĂPĂLOAGĂ, Bogdan GEORGESCU, Adela Ioana MUSTĂȚEA, Sorina Andreea MIHAI

The study describes the morphological characteristics of the panther leopard skull (Panthera uncia - Schreber, 1775). The skull particularities are valuable elements necessary for species identification. Following the analysis, the following conclusions emerged: the existence of an interfrontal fossa, elongated in an oro-aboral direction, located at the level of the interfrontal sagittal suture; the nasal extremity of the frontal ends with a sharp process; between the two processes of the rostral extremity of the nasals, lateral and medial, there is a wide and shallow incision; the naso-incisive incision is reduced; the mastoid process is reduced; a reduced accessory lacrimal foramen is present; between the maxillary and sphenopalatine foramen, an obvious vascular foramen can be observed; medio-aboral, on the edge of the jugular foramen is the hypoglossal canal; the pharyngeal tubercle is reduced and limited on the sides by two reduced fossae; the external occipital crest is well highlighted; above the ventral condyloid fossae are the deep and elongated dorsal condyloid fossae; the mental foramen is accompanied by an accessory mental foramen.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Bianca-Maria MADESCU, Alina Narcisa POSTOLACHE, Ioana BOLOHAN, Sorin Aurelian PASCA, Paul Corneliu BOISTEANU

The purpose of this paper was to highlight the results of the morphostructural characterization of Longissimus dorsi muscle tissue of the Aubrac breed, exploited in Romania. The present research focused on a population of 38 cattle, including both males and females, raised in a semi-intensive farming system. Longitudinal measurements (major and minor diameter, mean, DM/Dm ratio, length) as well as transverse measurements (diameter, area) of muscle fibres were conducted, and the results were statistically interpreted. In terms of determining the length of muscle fibres in the longitudinal section, significant differences between genders are observed. In males, the large diameter recorded values of 77.17 µm, while in females, it was 64.73 µm. Regarding the determination of the area of muscle fibres in cross-sectional analysis, significant differences were noted between males and females (in males, it's an average of 2226.83 µm2, while in females it's 1576.60 µm2). This shows that in the Aubrac breed, the area of muscle fibres in meat content can vary by gender. In conclusion, longissimus dorsi muscle is particularly important and valuable in the beef industry.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Georgiana Ancuta MISU, Roxana-Andreea MUNTEANU-ICHIM, Cristina Maria CANJA, Mirabela LUPU, Florentina MATEI

Sous vide (SV), a culinary technique that involves vacuum sealing food and cooking it at precise temperatures, has been celebrated for improving food's texture, flavor, and nutritional content. Integrating natural inhibitors is a crucial strategy for enhancing food safety, shelf life, and health benefits by fighting microbial growth. This review examines the combined effects of SV and natural inhibitors on various food types, including meats, seafood, and vegetables. It highlights significant progress in food safety, with natural additives helping to reduce pathogen resistance and curb bacterial spread, thus improving the overall safety and sensory appeal of meals. Moreover, it discusses the challenges and opportunities in refining the use of natural inhibitors in SV cooking, such as achieving the right balance between flavor and microbial management. The adoption of SV varies globally, reflecting its adaptability in different culinary traditions. The review suggests future research avenues, like exploring new natural inhibitors, cooking conditions, and their effects on consumer health, emphasizing SV's role in modernizing cooking practices to meet demands for quality, and safety.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVII, Issue 2
Written by Elena GHERASIM

The paper presents data on the identification for the first time in Moldova of the helminth fauna structure of Rana temporaria and the determination of its role as bioindicators and as vectors for parasitic agents specific to animals. As result of helminthological investigations during 2014-2023 years, 19 helminths species was established. The predominant group are trematodes with 57.8% of cases, nematodes with 31.6% of cases, acanthocephala and monogeneans with 5.3% of cases each. According to the assessment of the main helminthological indices, it was established that the species is infested with helminths in 96.3% of cases. When evaluating the data and the composition of helminth species, it was found that adult forms of helminths are predominant over larval forms, so that, when the host species is infected with trematodes, 9.1% are metacercariae, and 90.9% are the adult forms, when the host species is infected with nematodes 33.3% are the larval stages, and 66.7% are the mature forms, when infesting the host species with acanthocephals and monogeneans, it was established that the helminth species are an adult form, each constituting 5.3%.

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© 2019 SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE. To be cited: SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE.

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