ISSN 2285-5750, ISSN CD-ROM 2285-5769, ISSN-L 2285-5750, ISSN Online: 2393 – 2260
 

Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Marius Mihai CIOBANU, Paul Corneliu BOIȘTEANU, Mugurel MUNTEANU, Dumitru TÂRZIU, Roxana Nicoleta RAȚU, Alina Narcisa POSTOLACHE

Intense concern in recent years for quantitative and toxicological identification and evaluation, especially for heavy metals from animal origin products represent a side that needs to be studied continuously, especially for game meat, nutritional toxicity of those mineral elements (mainly Pb and Cd) being directly influenced by their variations in habitat water, air and soil. The goal of the study is motivated by the inexistence of information regarding appreciation of contamination degree with heavy metals of game meat, having in view the limited checkout of feeding of game animals exploited in their natural environment, as well as the polluting environmental factors. The analytical results regarding the quantitative evaluation of the Cd concentration of the muscle samples indicate that the average values found in male carcasses are higher than those in female carcasses, except the Trapezius cervicalis muscle. The average concentration of Cd residues ranged from 0.020 ± 0.0028 to 0.040 ± 0.017 mg/kg DM, both limits being attributed to the Triceps brachii muscles. The range of averages corresponding to the other muscle groups are described by an amplitude of the variation of 0.018 mg/kg DM.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by David POGHOSYAN

The use of the technology with feeding calves for beef based on a high-concentration type of feeding (59-61% of the energy nutritional value) on Aberdeen-Angus calves led to an increase in the meat productivity of animals in comparison with the control group kept under the traditional feeding system adopted for beef cattle "cow-calf". At the same time, during the period of growing and fattening (3-12 months), the average daily gain in live weight of bulls in the experimental group was 1359 g, which was 30% higher than these indicators in the control group, and was accompanied by an increase in the absolute gain in live weight of young animals by 81.4 kg as well as slaughter yield by 3.6%. The use of the technology with feeding calves for beef based on the high-concentration type of feeding (65- 70% of the energy nutritional value) on Holstein calves led to an increase in the meat productivity of animals in comparison with the control group kept under the traditional feeding system adopted for young dairy cattle with a level of concentrates in the diets more than 50%. At the same time, for the period of growing and fattening (3-12 months), the average daily gain in live weight of bulls in the experimental group was 1359 g, which was 47% higher than these indicators in the control group, and was accompanied by an increase in the absolute gain in live weight of young animals by 120 kg, as well as slaughter yield by 3.1%.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Lyudmila NIKOLOVA, Stanimir BONEV

Seven-year-old male (n = 25) and female (n = 25) Russian sturgeons (Acipenser gueldenstaedtii), grown under the same conditions on a super-intensive cage farm, 36 plastic and 7 meristic traits are studied and 7 morphometric indices are calculated. Female fish had significantly (P <0.001) better fattening than males, their body was more compact (P <0.001) and thicker (P <0.01). In females, the ratio of antiventral distance, back thickness and body girth to the total body length is higher. In male fish, the ratios of the head lengths, the caudal stalk, the ventral fin, the anal fin height and the ventro-anal distance to the total body length are relatively larger. In female fish, the height and length of the space behind the eye are greater, they have a wider mouth, a larger eye and a wider snout. In males, the long-headed index is higher (P <0.05), the snout and the distance from its end to the mouth are longer, the lower lip interruption is larger. Female fish have significantly fewer ventral bone scutes the left (P <0.05) and the right (P <0.01) sides of the body.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Diana Oana MIHAI (OPREA), Carmen Daniela PETCU, Dana TĂPĂLOAGĂ, Corina PREDESCU, Marian GHIȚĂ, Oana Mărgărita GHIMPEȚEANU, Otilia Cristina MURARIU, Emilia CIOBOTARU-PÎRVU

Stress is defined as a complex chain of events, consisting in a stimulus that causes a subsequent reaction in the brain and activates physiological reactions. It is important to adopt good practices during slaughter. In assessing the level of stress, the following variables should be taken into account: the means of transport, the way of slaughter (with or without stunning), accidental fall of animals, refusal of animals to enter the containment box, excessive movement of animals during containment. In the living organism, a series of biochemical and energetic transformations take place, which are in close interdependence, and are subjected to the mechanisms of regulation and metabolic control, which cease with the suppression of animal life, therefore, after slaughtering animals, a series of transformations appear in the muscle tissue. The study was conducted between 2019-2020, on two batches of conventionally slaughtered pigs (with stunning) in slaughterhouses and on a batch of traditionally slaughtered pigs (without stunning). In the slaughterhouses, the technological flow of pigs slaughtering was monitored and blood samples were collected in order to extract the serum and measure the cortisol level. Cortisol was measured in a specialized laboratory by the immunoenzymatic method by chemiluminescence detection. Determination of cortisol levels in blood samples taken from conventional pig slaughter revealed different values, exceeding the established reference values, compared with blood samples collected from households following traditional slaughter, the level of which is lower, sometimes falling within the reference values. The growth and handling of pigs before slaughter induces their stress, so special attention must be paid to the slaughter process in order to minimize stress levels and improve the quality of the meat.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Diana Anis SELVIE, Agnitje RUMAMBI, Siane RIMBING, Constantyn SUMOLANG

Indonesia is one of the larger producer copra in the world, so coconut still economic backbone of society. A tropical grass namely koronivia grass well adapted under mature coconuts, crude protein content lower than minimum requirement in the ruminant diet, carrying capacity able to provide only the needs of total digestible nutrient for cattle with bodyweight no exceed to 250 Kg. Needs inorganic nitrogen fertilizer application but costly and occurred negative impacts on the environment. Herbaceous legume integrated as mixed pasture failed to persist due to the aggressiveness of this koronivia grass. This research aims to study the ability of tree legume Indigofera zollingeriana as a source of protein and dry matter to enhance the carrying capacity. Koronivia grass solely could provide dry matter, crude protein and TDN for cattle with body weight 250 Kg, and I. zollingeriana as well, but the latter provides crude protein more, almost double than koronivia. Both species altogether in the same space and management could provide feeds to fulfil the requirement of cattle more than 250 kg body weight.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Marius Gheorghe DOLIȘ, Claudia PÂNZARU, George Marius DOLIȘ, Marius MAFTEI, Roxana Nicoleta RAȚU

The paper presents the results of a study accomplished in the Rădăuți stud farm, which aimed to analyze some morphological and reproductive aspects of Shagya Arabian horses. The selected population was represented by broodmares from 2000y generation, introduced in the reproductive herd until now. The average value of height was 159.188 ± 0.593 cm, the average of heart girth was 177.188 ± 1.013 cm, and the cannon perimeter was 18,594 ± 0,184 cm for the studied horses. Regarding the reproduction aspects, it was revealed that the studied parameters had the following average values: 4.7 ± 0.3 years for age of introduction to reproduction, 4.5 ± 0.2 years for the age at first foaling, 338.7 ± 0.9 days for the gestation length, 149 ± 18.3 days for the service-period parameter, 503 ± 19.9 days for the foaling-interval parameter, and 14.6 ± 1.2 years for the reproductive longevity of broodmares. The conclusion was that all data obtained fall within the normal limits specified by the literature.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Aida ALBU, Simona-Maria CUCU-MAN, Cristina-Gabriela RADU-RUSU, Ioan Mircea POP

Twenty-four samples of monofloral Romanian honey (acacia, linden, rapeseed and sunflower) were analysed for their total phenolic and flavonoid contents, pH, free acidity, ash, electrical conductivity and color intensity. The analyses were performed in accordance with Romanian and EU standards and according to the methods in the literature. The results for color varied between 0.9-69.1 mm Pfund, for pH and free acidity between 3.54-4.44 and 4.7-15.7 meq kg-1, respectively. The values of ash and electrical conductivity were between 0.043-0.291% and 0.12-0.55 mS cm-1. The total phenolic content ranged from 14.50 mg GAE/100 g to 30.13 mg GAE/100 g while total flavonoid content ranged from 0.59 mg Q/100 g to 2.84 mg Q/100 g. The Pearson correlation analysis indicates positive significant correlations between color and total flavonoid content, ash and electrical conductivity, ash and total polyphenols content.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Angelica DOCAN, Maria Desimira STROE, Iulia GRECU, Lorena DEDIU, Mirela CREȚU, Magda TENCIU

Fish parasites represent a major part of aquatic biodiversity. The aim of present investigation was to assess the diversity and distribution of parasites from some fish species from the Prut River. Fish were sampled from three station on Prut River (Rădăuți, Drânceni, Oancea) during the year 2020. The different types of fishes from 5 families Cyprinidae, Esocidae, Percidae, Siluridae, Cobitidae have been examined for analysis of the distribution of parasites from these fish, in order to complete the existing data on metazoan parasites of freshwater fishes in the Romanian sector of Prut River. Parasitological investigations were performed on fresh samples by classic methods and the obtained results were expressed in grades of prevalence and intensity. In the analysed fish, were identified 12 parasitic species belonging to 6 systematic groups: Protozoa, Monogenea, Trematoda, Cestoda, Nematoda, Anelida. The prevalence of the parasitosis varied among examined fish species. The ciliated protozoa and monogenic worms were the most commonly identified parasites, but the intensity of parasitism was low. The effects of parasites on fish hosts in the natural environment may be difficult to isolate and quantify.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Aleksei Zagumennov, Valeriy Ermolaev, Alexander OSTAPCHUK, Lilia OSHKINA

Ophthalmopathology in young cattle is one of the reasons for the decline in the growth and development of calves. The cause of keratoconjunctivitis is very often a different microflora. Economic losses in keratoconjunctivitis are formed due to a slowdown in the growth and development of young animals, a decrease in productivity, and a loss of live weight. 25-30% of recovered animals remain blind, the same number lose sight by 50%. Determination of the protein spectrum and immunoglobulins in the blood of calves is of great diagnostic and prognostic value, which reflects the degree of intensity of metabolic processes and the level of nonspecific resistance of the organism. Serum proteins are components of a dynamic circulating system and reflect the physiological and biochemical characteristics of the body as a whole. They take part in tissue nutrition, the formation of immunity in infections and invasions (γ-globulin is a fraction of serum globulin, which consists mainly of immunoglobulin antibodies), maintaining pH and osmotic pressure. In ophthalmic diseases, there is a violation of the ratio of plasma protein fractions (dysproteinemia).

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 2
Written by Dawar Hameed MUGHAL, Ijaz AHAMD, Muhammad Shehbaz YOUSAF, Umer FAROOQ, Fazal WADOOD, Amjad RIAZ

The present study was designed to: 1) study the influence of various concentrations of trehalose on buffalo bull spermatozoa extended in lactose- egg yolk-glycerol extender (LEYGE) and 2) compare the fertility rate between LEYGE supplemented with an optimal level of trehalose and traditionally used tris-citric-acid-fructose extender. Semen of Nili- Ravi buffalo bulls (n = 04) routinely used for artificial insemination, was collected using an AV at SPU once a week, for eight weeks. Semen samples with ≥70 % spermatozoa motility were pooled and diluted at 37°C in LEYGE extender containing trehalose at 0.0, 30.0, 50.0 and 70.0 mM. The sperm motility, viability, acrosomal integrity, plasma membrane integrity, and DNA integrity were significantly (P≤0.05) higher in LEYGE supplemented with 70.0 mM trehalose as compared to other groups. The fertility rate was significantly (P≤0.05) higher when semen doses extended in LEYGE and supplemented with 70.0 mM trehalose were used (n = 19; 38%) as compared to semen doses extended in the conventional tris-citric-acid-fructose extender (n = 27; 54%). In conclusion, 70 mM is an optimal inclusion level in buffalo bull semen for trehalose in LEYGE in respect to seminal attributes. Furthermore, LEYGE supplemented with 70 mM trehalose presented higher fertility rate in buffaloes as compared to traditionally used tris-citric-acid-fructose extender under field conditions.

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© 2019 SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE. To be cited: SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE.

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