ISSN 2285-5750, ISSN CD-ROM 2285-5769, ISSN-L 2285-5750, ISSN Online: 2393 – 2260
 

Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Daniela-Mihaela GRIGORE, Narcisa Elena POGURSCHI, Ionuţ RANGA, Narcisa BĂBEANU

Vitamin A is an essential nutrient, for both production and reproduction of farm animals, however, most animals are unable to synthesize de novo precursors of vitamin A (β-carotene), and dietary supplementation is mandatory. The current paper aims to evaluate the analytical parameters and to improve the method efficiency for determining β-carotene content in red yeast and the internal laboratory validation protocol. Our method stands for the ultraviolet-visible spectrophotometric uses, having the Lambert-Beer law as the basis. To determine the β-carotene content, dimethyl-sulfoxide was employed as a solvent, calibration curve, and visible spectra were evaluated (300-900 nm). The linearity of beta carotene measured at 465 nm using the UV-Vis method linearity range was 6.1-36.6 µg/ml, R2=0.997, LOD = 5.39 µg/ml, LOQ = 16.32 µg/ml, SD less than 5% and RSD in between 1-15%. In conclusion, the β-carotene spectroscopic method determination is a cheap, and efficient method, suitable for β-carotene determination and retinol and retinoic acid estimation for nonconventional feed additives such as yeasts.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Vera GRANACI, Valentin FOCSHA, Alexandra KONSTANDOGLO, Vasily CURULIUC, Valentina CIUBATCO

The study results of the reproductive ability of cows depending of their age and milk productivity are presented. The studies were conducted in the Republic of Moldova on cows of the Holstein breed. The optimal parameters for the duration of the SP and CI were detected in cows with milk productivity less than 6000 kg of milk, at which the animals were characterized by the maximum indicators of calves yield and CCR. In cows with a milk yield of 9001 kg or more, a significant increase in the duration of SP (+109.8 days) and the CI (+90.3 days) was observed, the CCR decreased by 0.22, and the yield of calves/100cows per years by 21.7 heads. On average, each increase in milk yield by 1000 kg increases the duration of the SP by 27.6 days, the CI by 20.9 days. The share of the impact (ɳ2x) of level of milk yield on the duration of SP was 18.0% (p<0.001), on the duration of the CI 15.6% (p<0.001), on the CCR 25.6% (p<0.001), the yield of calves 35.6 % (p<0.001). In order to increase the economic profit, we recommend livestock holdings to monitor the duration of SP.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Sonia BEN FRAJ, Dănuț Nicolae ENEA, Monica MARIN, Gheorghe Emil MĂRGINEAN, Livia VIDU

This paper focuses on the dynamic of milk protein in cows and sheep and explores the factors that influence milk protein content. Milk protein plays a crucial role in determining the quantity and quality of milk products, and therefore, various studies have been carried out to investigate the factors that influence milk protein content. To achieve this, we plan to monitor several farms raising different breeds of cows and sheep over a period of time to determine the changes of protein content. The breeds of cows that will be monitored include Holstein, Brown, and Romanian Spotted breeds. While the breeds of sheep that will be monitored have not yet been determined. Previous research has shown that optimizing the nutrition and management of cows and sheep can increase milk protein content. However, further research is necessary to better understand the complex dynamic of milk protein in both species and to develop best practices for improving milk production and quality.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Oana Diana MIHAI, Simona NICOLAE (CĂLIN), Alina-Daniela MARCU, Diana-Nicoleta RABA, Iuliana CODREANU, Emilia CIOBOTARU-PÎRVU

The purpose of this study was to evaluate the influence of the administration of nutritional supplements on the quantity and quality of goat’s milk. For this purpose, homogeneous groups of goats from the Carpathian breed, in the middle of the lactation period (n = 10), were created, to whom were administered in food, experimentally: hemp seeds (group 2), hemp seeds and mineral supplement (group 3), flax seeds (group 4), flax seeds and mineral supplement (group 5). Milk production and milk protein and fat percentages were monitored for 20 days, and the results obtained were compared with those of the control group (group 1). The obtained results showed that in terms of milk production, there were significant increases (p<0.05) between the control group and the experimental groups (with 6.95% for group 3 and by 5.42% for group 5). Regarding the percentage of proteins, significant increases were observed in the case of groups 3 (6.06%) and group 5 (9.09%). Regarding the percentage of fat, it increased in the case of all experimental groups, the increases being 4.65% (p<0.05) for group 2, 11.62% (p<0.01) for group 3, 6.97% (p<0.05) for group 4, and 9.30% (p<0.01) for group 5.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Cristina ȘURLEA (ȘURLEA-STOICA), Cristina MATEESCU, Georgeta DINIȚĂ, Iuliana CRÎNGANU, Carmen Georgeta NICOLAE

At it is known, the development of larvae from worker bees or queen bees is identical during the first two days of the larval stage. Morphological differences appear starting from the third day of larval development. The food administered to the larvae ex-situ, at regular intervals, forms a dry film upon contact with the larva, under conditions of temperature and humidity similar to those in the hive. The food drying phenomenon is due to the temperature-humidity variation, in the hive these parameters are regulated and monitored by the nurse bees. In the study, different combinations of temperature and humidity values in the controlled environment were tested, so as to increase the number of queens obtained, by increasing food intake. As a conclusion, at a temperature between 35-36ºC and a relative humidity between 75% and 85%, it is possible to obtain 20-25% queens that reach maturity from total number of two days old age larvae from the start of study.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Ion RĂDUCUȚĂ, Costică CRISTIAN, Vlăduț Dragoș BULMAGA, Andra-Georgiana PAIU, Andrei MARMANDIU, Ion CĂLIN

The aim of the present work was to analyze the lactogenic potential in the second lactation of the F1 crossbreeds resulting from crossing the local sheep from the north-eastern area of the country with Awassi breed rams. To determine the total milk production, the control of milk production includes the suckling period of the lambs and the milking period of the ewes. The AT4 method was used in the milking period following the technical specifications recommended by the ICAR. During the suckling period, the amount of milk in crossbreds F1 sheep was 49.72 kg, and the production of milked milk was 95.13 kg. Average daily milk production on the 4 controls for F1 crossbred ewes was 774.37 ± 9.76 g with limits between 542 and 1006 g milk. The total production of milk obtained in 180 days at the second lactation of crossbreds F1 sheep was 144.85 kg, being 3% lower compared to that obtained by purebred Awassi sheep. The results regarding milk production at the second lactation of the F1 crossbred ewes are very good, so we recommend to breeders the use of crossing local sheep from the north-eastern area of the country with the Awassi breed for a significant improvement in milk production.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Lucica NISTOR, Paula POȘAN, Camelia HODOȘAN, Andra ȘULER, Marius MAFTEI, Şerban PURDOIU

The paper proposed an analysis of the physico-chemical composition of several types of grape varieties (white and red). Their analysis was done during the years 2019-2021 at a winery in Prahova County. The determinations were made on must, and the analyzed varieties were: Sauvignon Blanc, White Fetească and Black Fetească. The sugar content was determined, which is an important parameter in wine-making to estimate the favorable time for harvesting, but also to decide what wine is produced (dry, sweet, semi-sweet). The total acidity of the wine was also determined, acids representing important components of the wine. Low acidity will cause a "flat" and uninteresting taste, and too much acidity will cause an overly sour and astringent taste. At the same time, the pH was analyzed, which affects the taste, sugar, acidity level and stability of the wines. The observed values were in normal limits.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Camelia HODOȘAN, Lucica NISTOR, Andra ȘULER, Sorin Iulius BARBUICA, Raluca Ioana HODOȘAN, Ana Maria NEGULEI

This study aimed to monitor the quality of food products distributed in fast food restaurants. Physico-chemical and microbiological analyses were conducted on burger and sandwich products containing beef, chicken, turkey, fish, cheese, as well as on sweet desserts such as apple juice, ice cream, and milkshakes. Additionally, the equipment and utensils used in the preparation of these products were checked microbiologically. The ice and drinking water from the supply network, used in the preparation process of these products, were tested both microbiologically and physic-chemically.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Georgeta DINIȚĂ, Marius Gheorghe DOLIȘ, Anca GHEORGHE, Mihaela HĂBEANU, Teodor MIHALCEA

One of the objectives of the sericultural research is the reducing of research field was the reducing of chemical fertilizers quantity by applying some ecological agricultural practices, among which it can be mentioned, next to using extra-radicular fertilizing, also the biofertilizers of vesicular-arbuscular mycorrhize (VAM) type, in the form of commercial products obtained by biotechnologies of high biological performance. The biofertilization of VAM type aims to reduce or eliminate the chemical fertilization, the mulberry being a plant with a high consumption of mineral elements. Also, this type of mycorrhize stimulates the plants growing and development, having a role in soil remediation and nutrition improvement from soil-plant system.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXVI, Issue 1
Written by Bogdan TAȘBAC, Tiberiu BURINARU, Gavrilă ZAGRAI, Otilia Cristina MURARIU

Currently, immunomodulation is an alternative in the fight against many diseases, being considered a possibility to fight against many infectious diseases that can affect pig herds. Selenium can be used for this purpose, the effect of its administration on the immune response being the main purpose of the present study. Following the administration of organic selenium, we found that in the case of WBC parameters, granulocytes and agranulocytes percentages and lymphoblastic transformation percentages of B lymphocytes, there are no major differences between the values recorded at the beginning of the experiment and the values recorded in the two experimental moments. Instead, following the administration of organic selenium, we observed significant increases in T lymphocytes percentages (by 9.94%, after 21 days, and respectively by 8.18%, after 30 days), percentages of lymphoblastic transformation of T lymphocytes (by 59.59% after 21 days, and respectively by 64.14% after 30 days), as well as the helper T lymphocytes/ supressor T lymphocytes ratio (by 46.15% after 21 days, and respectively by 65.38% after 30 days). Regarding the percentage of B lymphocytes, a decrease of this parameter is observed by 38.34% after 21 days and by 21.84% after 30 days following the administration of the product based on organic selenium.

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© 2019 SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE. To be cited: SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE.

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