ISSN 2285-5750, ISSN CD-ROM 2285-5769, ISSN-L 2285-5750, ISSN Online: 2393 – 2260
 

Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Anca Nicoleta CORDELI (SĂVESCU), Mirela CREŢU, Lucian OPREA

This experiment aimed to determine the optimum dietary protein level for common carp fry (Cyprinus carpio L.), with the average initial body weight of 2,98 g ±0,05 g (W) and the total length of 5,42±1,17mm (TL). The experiment was conducted for 31 days in a recirculating aquaculture system (RAS). The fish were divided into four experimental groups, in duplicate: V1-30% crude protein and 7% lipids, V2-44% crude protein and 22% lipids, V3-45% crude protein and 16% lipids, and V4- 50% crude protein and 14% lipids.Weight gain (WG) and SGR significantly increased (p<0.05) as dietary protein levels increased.Feed conversion recorded the best values at 50% protein diets, the values being considerably lower than those from V1, V2,and V3 variants. Based on the obtained data, it was estimated that the optimal level of protein for fry carps weighing between 2,9 g and 7 g was 50%.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Lavinia IDRICEANU, Nicoleta Aurelia LEFTER, Anca GHEORGHE, Mihaela HABEANU, Dumitru DRAGOTOIU

At present, the widespread raising of swine in farms becomes a serious problem in terms of the emission of carbon dioxide (CO2), having an environmental impact. The objective of this study was to investigate the effect of different diets on growth parameters changes in physical size, carbon dioxide (CO2) emission and microclimate monitoring. For 28 days, 7 TOPIGS sows (average initial body weight, BW ~ 205.09 kg) were allotted in two dietary treatments: control (C diet, 1.6% soybean oil) and experimental (E diet), (with 1.6% hemp oil). The type of oil differed by their fatty acids (FA) composition. Sows were individually weighed, P2 backfat thickness was determined by ultrasound and morphometric measurements of body size were taken three times during lactation. The CO2 emitted by sows and their litter was calculated. Microclimate parameters were recorded daily. The addition of 1.6% hemp oil in the diet improved sows final body weight and reduced weight loss (P<0.05). A high significant negative Pearson correlation was observed between PUFA intake, and their fraction (Σ n-3, Σ n-6, Linolenic and Linoleic as predominant n-3 and n-6 FA). The proportion of CO2 exhalated by sows fed E diet was significantly lower compared to C diet. The CO2 emission, temperature and relative humidity were affected (P<0.001) by the sows’ physiological status. The results from this study are indicating that the hemp oil inclusion in the sow's diet has a beneficial effect on performance. Further studies are necessary to determine the nutrition impact on environmental conditions.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 2
Written by Minodora MANU, Constantin-Ciprian BÎRSAN, Owen MOUNTFORD, Anca Rovena LĂCĂTUŞU, Marilena ONETE

The research objective was to demonstrate the use of soil fauna groups as a tool for monitoring of the “Springs Complex of Corbii Ciungi” protected area (the IUCN category 4), Dâmboviţa County, Romania. Some ecological indicators were quantified: taxonomic diversity, numerical abundance, dominance, evenness, equitability, Acari/Collembola ratio; Oribatida+Mesostigmata/Prostigmata+Astigmata ratio; correlations between the investigated communities and environmental factors (soil layer thickness, air temperature and humidity, soil temperature and moisture, soil pH, soil penetration resistance, exposure, slope, amount of organic carbon, total N, soil nutrients and the vegetation cover). Transects located near the water sources, were characterised by biological indicators with higher values. There was a significant interdependence between environmental variables. They influenced the abundance and distribution of the edaphic taxonomic groups. Statistical multivariate analysis showed that certain edaphic groups (from a total of 34 taxonomical taxa) are dependent especially on soil moisture e.g. Lumbricidae, Collembola and Oribatida.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 1
Written by Florica BUȘURICU, Verginica SCHRODER, Doina MARGARITTI, Dorina NADOLU, Andreea Hortanse ANGHEL

Legislation enforce allows the use of food additives that have adverse effects on the human body, such as: asthma, contact hives, allergies, digestive disorders, ADHD, cancer. The toxic effect is particularly enhanced when more than one additive is associated in a food. In this context, we propose to study the effect of benzoate ± sorbate on some food colorants (E129 red allura and E133 bluish blue) from 8 fruit juice samples. Sodium benzoate was dosed by the spectrophotometric method using a standard solution Cs = 0.1 mg/mL. Evaluation of the cytotoxicity of these juice additives was followed using the BLSA (Brine shrimp lethality assay) test. For all analyzed samples, the maximum admissible value for benzoate (200mg/L) is observed to be followed. The fastest cytotoxic effects were recorded in the first 24 hours, but at low concentrations of 50 μL/mL and 100 μL/mL. Processes such as cytoplasmic accumulation of inclusion vesicles, disruption of membrane activity, as well as phenomena associated with cell division and differentiation are identified. In conclusion, although all products contain benzoate levels within acceptable limits, there is a risk of accumulation of larger quantities in the body, by consuming products with this preservative. The combination of these preservatives with dangerous dyes increases the toxic potential, which was also confirmed by the BSLA test. Proper product labeling would inform and guide the consumer / patient to balanced consumption with fewer synthetic additives, with the option of choosing an alternative that does not harm the health.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIII, Issue 1
Written by Elena IRIMIA, Daniela-Mihaela GRIGORE, Ioana NICOLAE, Dinu GAVOJDIAN, Stelian BĂRĂITĂREANU, Livia VIDU

In order to study the influence of calves sex, dams age and year of calving, it is very important to evaluate at farm level, the incidences of colibacillosis, coccidiosis, rickets, neonatal enteritis, respiratory diseases (BRD) and haemorrhagic enteritis in un-weaned dairy calves. The study was carried out at the dairy cattle farm of the Research and Development Institute for Bovine Balotesti, Romania between January 2018 and December 2019, with health datasets from 176 Romanian Black Spotted calves. Colibacillosis and coccidiosis incidences were not influenced (p>0.05) by the sex of the calves, while rickets incidence was significantly influenced (p≤0.001), with 2.20 ± 1.55% of the male calves being affected and 10.59 ± 3.36% of the females, respectively. Year of birth had significantly influenced (p≤0.05) the incidence of colibacillosis and rickets and had no effects (p>0.05) on coccidiosis incidence. Neonatal enteritis, BRD and haemorrhagic enteritis incidences were not influenced (p>0.05) by factors such as calves sex, dams age and year of birth. Colibacillosis and rickets incidences were the only health problems influenced by factors such as calves sex and year of birth.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Doina ROTARI, Grigore DARIE, Oleg CHISELITA

The research was performed on ram semen. The biological material was the Moldovan Karakul rams. Ejaculates with a mobility of over 70% and a sperm concentration of over 2 billion / ml were allowed for processing. The basic medium used was SȚJ (sucrose, sodium citrate, egg yolk). An additional component introduced in the basic environment was experienced the biologically active preparation LB / MP obtained from yeast from the brewing by the Institute of Microbiology and Biotechnology of the Academy of Sciences of the Republic of Moldova. After diluting and microscopically testing the sperm using the CEROS computer system, the experimental batches were introduced into the cold room at + 2-4 degrees. Mobility assessment was performed at 24 hours intervals. Sperm mobility after 120 hours of storage by refrigeration was maintained at 68.0 ± 5.8 % in the experimental group where the concentration of the preparation in the base medium was 0.8%, mobility allowed for inoculation. Based on the experimental results obtained, it was proposed to dilute and preserve ram semen by refrigerating the SȚJ medium supplemented with 0.8% biologically active preparation LB/MP.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 1
Written by Doina ROTARI

Were studied quantitative and qualitative indicators of sperm obtained from rams Karakul Moldovenesc. The average volume of the ejaculate was 0,84 ml, 91% mobility and 2,5mlrd / ml concentration. The action of the BD-1 preparation synthesized at the Institute of Microbiology and Biotechnology of the Academy of Sciences of the Republic of Moldova was experimented as an additional component introduced into the basic environment regarding the quality of the ram seminal material, preserved at 2-4 ° C. It has been found that the introduction in the composition of the basic medium for 6% dilution of seminal material of BD-1 has a positive influence on the preservation of the semen. After 144 hours of incubation at 2-4 °C the sperm mobility was 68% compared to the control group where this index was 45%. artificial insemination of the sheep with refrigerated sperm after 144 hours allowed 71,9% fecundity sheeps. The proposed technology for conserving the semen of ram at 2-4 °C which composition proposes the dilution medium for semen, containing: glucose, sodium citrate, egg yolk, BD-1, allows the results of artificial insamination of sheep to be made more efficient.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 2
Written by Doina ROTARI

Quantitative and qualitative indicators of sperm obtained from rams Moldavian Karakul were studied. The averagevolume of the ejaculate was 0.84 ml, 91% mobility and 2.5 mlrd / ml concentration. The action of the BD-1 preparationsynthesized at the Institute of Microbiology and Biotechnology of the Academy of Sciences of the Republic of Moldovawas experimented as an additional component introduced into the basic environment regarding the quality of the ramseminal material, preserved at 2-4°C. It has been found that the introduction in the composition of the basic medium for6% dilution of seminal material of BD-1 has a positive influence on the preservation of the semen. After 144 hours ofincubation at 2-4°C the sperm mobility was 68% compared to the control group where this index was 45%. Artificialinsemination of the sheep with refrigerated sperm after 144 hours allowed 71.9% sheep fecundity. The proposedtechnology for conserving the semen of ram at 2-4°C which composition proposes the dilution medium for semen,containing: glucose, sodium citrate, egg yolk, BD-1, allows the results of artificial insemination of sheep to be mademore efficient.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXIV, Issue 1
Written by Mihaela DUMITRU, Mihaela HABEANU

The production of different enzymes, including amylase and protease by Bacillus licheniformis ATCC 21424 were tested on different raw materials and compound feed, which were used as a substrate in process fermentation: soybean meal, peas, sorghum flour, corn and compound feed (FC). The bacterial growth and enzyme production were done in a fermentative medium (Erlenmeyer 100 ml flash in a shaking incubator) and enzyme activity was registered after 24, 48 and 72 h, pH 7 ± 2). The inoculum strain presents 10.19 log CFU/ml at 37°C, 24 h, 150 rpm. The screening showed a capacity of amylase and protease strain production. The highest amylase activity was obtained when the strain was cultured in corn fermentation medium (19.43 U/ml), followed by soybean meal (18.31 U/ml), sorghum (17.52 U/ml), peas (19.43 U/ml), and FC (6.63 U/ml) at 72 h. Great protease activity was noticed in FC (97.75 U/ml), soybean meal (94.67 U/ml), sorghum (89.36 U/ml), corn (78.6 U/ml), peas respectively (75.91 U/ml). The observation of this study suggested that Bacillus licheniformis ATCC 21424 could be capable of producing protease and amylase enzymes, particularly in fermented medium contained soybean meal or corn, and can be administrated in animal nutrition as source of feed additive.

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Published in Scientific Papers. Series D. Animal Science, Vol. LXII, Issue 1
Written by Ion Tudor COMAN, Livia VIDU, Monica Paula MARIN, Georgeta ȘTEFAN, Gheorghe Emil MĂRGINEAN

The paper aims to carry out an external analysis of Romania's proposal to regulate the regulatory framework in the field of milk production and processing, to establish the way of marketing of dairy products, to increase consumer confidence in domestic dairy products, to eliminate falsified products from the market dairy products, to facilitate the identification of authentic products by consumers and, last but not least, to the increase in milk production due to all the measures mentioned.

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© 2019 SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE. To be cited: SCIENTIFIC PAPERS. SERIES D. ANIMAL SCIENCE.

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