Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Valentin FOKSHA, Aleksandra KONSTANDOGLO, Gregory MORAR, Gennady PEYKOV , Vasily KURULYUK, George TATARU

The scientific paper presents the results of research of productive and reproductive parameters of cows of Holstein breed of Dutch and German origin in the herd J-S.C. „Aydyn”, Comrat Administrative and Territorial Unit Gagauzia. For the analysis was used performance control for the year 2015: cows on the first lactation (n=122) and the second lactation (n=43) of Dutch origin and cows on the first (n =129) lactation – of German origin. It was established that from cows of the Dutch origin for the first lactation on average was received 7803 kg of milk, which is with 589 kg more than from the cows of German origin, a highly significant difference (P <0.001). It is established by the analysis that 50,8% of the animals of Dutch origin had the productivity from 7501-8000 kg to 9001 kg, whereas 70,5% of the animals of German origin had the productivity from 6000-6500 to 7001-7500 kg and only 29.5% from 7501-8000 to 9001 kg of milk per lactation. The average length of the dry period at cows of Dutch origin was by 6.5 days less than at the cows of German origin, the difference is authentic at P <0.05. Service period at cows of Dutch origin averaged 219.9 ± 12 days, the German origin – 168.2 ± 15.4 days. This affected the increase of the between calving interval at the animals of Dutch origin (483 days), the German origin (446 days), which exceeds the desired duration by 118 and 81 days, respectively.

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Published in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Natik H. ALKUDSI, Duaa A. HUSSEIN

Lactoferrin is a biologically active glycoprotein of the transferrin family found mainly in milk and to a lesser extent in other biological fluids as well as the secondary granules of neutrophils. As an iron-binding molecule, Lactoferrin is involved in the transport and excretion of iron but also known to bind to proteins such as IgA, casein, lysozyme and to DNA. This study aimed at establishing the relationships between Lactoferrin in cow milk and the concentration of minerals and amino acids. Ten Holstein Frisian cows in their first lactation were used in this study. Colostrum sample were collected immediately after parturition and milk samples were collected weekly for the first 60 days of lactation. Concentrations of minerals and amino acids in colostrum and milk samples were determined and used to construct the relationships between these variable and Lactoferrin concentration. The concentration of Lactoferrin in the colostrum was higher (P<0.05) than in milk (732.8 vs 350.3mg /L). The concentration of Lactoferrin in milk declines with advancing lactation. The concentrations of some minerals (calcium, phosphorus, magnesium and iron) in colestrum were 130 mg / L, 0.82%, 76.00 mg / L and 16.52 ppm respectively. The correlation coefficient between the lactoferrin and calcium, phosphorus, magnesium were positive and signifecant namely, 0.665, 0.268 and 0.289 respectively. The correlation coefficient between lactoferrin and iron was negative and significant (- 0.614) . Moreover, the correlation coefficient between Lactoferrin and the methionine and Lucien were positive and highly significant (0.18125 and 0.33908 respectively), While with the non-essential amino acids was being positive and significant. It conclusion, lactoferrin has a tight relationship with main milk components, being has an important role for birth calves through its high percentages in colostrum. Also, the repeatability between lactoferrin and minerals has a pronounced importance for birth calves growth.

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Published in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Ion BALAN, Gheorghe BORONCIUC, Nicolae ROŞCA, Vladimir BUZAN, Ion MEREUŢA, Iulia CAZACOV, Melania BUCARCIUC, Grigore VARMARI

The method of long-term preservation of mammalian semen in deep-frozen condition provides great opportunities for development and improvement of the reproduction system of farm animals. Using this method it is possible to check the breeders on the quality of offspring so as to maximum use the improvers. This allows performing the large-scale genetic selection in animal husbandry, which significantly increases the rate of mass improvement of breeding and productive qualities of animals. However, the existing cryotechnology not provide maximum preservation of the biological integrity of the reproductive cells. Comprehensive research has shown the possibility of increasing the efficiency of cryopreservation by improving of synthetic mediums and the development of optimum cryopreservation process parameters.

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Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Laurentius RUMOKOY, Sri ADIANI, Charles KAUNANG, Wisje Lusia TOAR, Hengky KIROH

This experiment was conducted to evaluate the influence of crude salive gland extracted from Stomoxys calcitrans applied to twelve young horses traditionally maintained. The treatment in study realized by injecting 0.5 ml of SGE to the foals compared to the control group of animals without SGE treatment. Each group was divided into two sub groups received colostrum and the other did notreceived any IgG colostrum. The IgG level was 60 gr/L colostrum and distributed to the foals with 9.6 gr IgG– 1J. The injection of SGE realized at the first day of experiment. The volume of blood collection was 3 ml through vena jugulaire at the 14th days after SGE injection. As soon as possible after collection, the blood was centrifuged and then its serum was placed in micro-tube to be observed. We did not found interaction of SGE and IgG on imunoglobuline-G serum (P>0.05) while the IgG serum level increased very significantly (P<0.001) as the influence of single treatment of SGE as described in data of A2B1 and A2B2, compared to the control without SGE.In other side this study showed an important relation between entomology and animal husbandry especially to the health care improvement for the young animals by using the antigen substance of the insect (Stomoxys calcitrans).

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Published in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Eli SAHARA, Tuti WIDJASTUTI, Roostita L. BALIA, ABUN

The aim of the study is to examine the effects of chitosan in the ration on Tegal duck performance. The dose of chitosan used ranging from 0.0%, 0.5%, 2% and 2.5%, mixed into the basal ration. The basal diet used iso-protein and isoenergy, with protein content of 15.34% and Metabolic Energy 2809 kcal / kg (NRC, 1994). Parameters measured were feed intake, duck day production (DDP), total egg weight and feed conversion. This study uses a completely randomized design (CRD) consisting of 4 treatments and 5 replicates and each replicate consisted of two ducks. The basal diet (R0) = without chitosan as a control, R1 = 0.5% chitosan, R2 = 2% chitosan, and R3 = 2.5% chitosan. Data were analyzed using SPSS 16 statistical program (Statistical Package for Social Science). Results indicated that chitosan showed no significant effect (P> 0.05) on feed consumption, duck day production, total egg weight and feed conversion. In this study, treatment of chitosan 2.5% (R2) gives the best results on day duck production, total egg weight and feed conversion. From the daily egg production (DDP), treatment R2 has a result of 59%, larger than R0 (57.76%), R1 (44.1%) and R3 (46.9%). Total egg weight for R2 = 3597.7 (73.42 g / grain) also show a greater number than the treatment R0 (2769.72), R1 (2662.46), and R3 (3403.14). On feed conversion, R2 showed the smallest (1.93) compared to R0 (3.06), R1 (7.4) and R3 (2.31). This means duck in treatment R2 more efficient of feed consumption, 1.93 kilograms to produce one kilogram of eggs.

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Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by An-An YULIANTI, Kurnia A. KAMIL, Diding LATIPUDIN, Darmawan DARWIS

The purpose of this study was to determine the effect of chitosan on the Pb content in the liver and bone of quail exposed to Pb-acetate in drinking water. Quail was kept in experimental cage of Poultry House, meanwhile, slaughtering and sampling were conducted in the Laboratory of Animal Physiology and Biochemistry, Faculty of Animal Husbandry, Padjadjaran University; furthermore, samples were analyzed in the Laboratory of Dairy Cattle, Bogor Agricultural Institute. The Experiment based on an experimental design with five treatments and five replications. Chitosan was given in ration as follows P0 (0 ppm), P1 (50 ppm), P2 (100 ppm), P3 (150 ppm) and P4 (200 ppm). Orthogonal polynomial test was used to see the trend of increased or decreased as a result of treatment response. The result of this study showed decreased levels Pb in liver and bone of quail along with increasing doses of chitosan given. The best result obtained at P4 (200 ppm) with the lowest Pb content in liver and bone of quail.

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Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Husmy YURMIARTI, Tri Anggarini Yuniwati FOENAY, Kusmajadi SURADI

New Zealand White crossbreed rabbit is a producer of meat and white pelt. Housing capacity and rabbitry area can affect production performance due to limited space allowance. The rate of growth of the male rabbit body weight tends to be faster than females, but is more easily experiencing stress due to its natural aggressivity, especially when kept in a cage with limited space that will ultimately have an effect on the quantity of pelt. The research aim was to study the influence of the density of cage and sex toward pelt quantity of New Zealand White Crossbred rabbit. The research used randomized complete block design with a 3x2 factorial pattern and four groups of rabbit at weaning weight as replicates. The first factor was stocking density (K1:1 rabbit/0,2 m₂, K2: 2 rabbits/0,2 m₂, K3: 3 rabbits/0,2 m₂), and the second factor was sex (S1: male, S2: female). A total of 48 crossbreed rabbits of New Zealand White aged 42 days were used in this research, consisted of 24 males and 24 females. Data were analyzed with analysis of variance followed by Duncan's multiple range test. Variables observed were quantity of pelt: pelt weight, pelt thickness, and pelt area. The results showed that there was no interaction between stocking density and sex in all parameters observed. Stocking density had an effect on pelt area, but not on pelt weight and thickness. Meanwhile, sex has an effect on pelt weight, but not on pelt thickness and pelt area. The conclusion is that the male rabbit pelt is better compared with females whilst density of 1 heads/0.2 m2 is better than 3 heads/0.2 m2 and 2 head/0.2.

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Published in Scientific Papers. Series D. Animal Science, Vol. LX
Written by Alex Kristian MANIK, Lovita ADRIANI, Diding LATIPUDIN

This research is aimed to determine the effect of giving fermented milk, soymilk, and thecombinationof it on meat cholesterol and intestine pH of broiler. The research was conducted on December, 19th2016 to February, 16th 2017 at Cipacing Village, Jatinangor, Sumedang, West Java, Indonesia. Thisexperiment uses a Completelty Randomized Design (CRD) in five kinds of treatment. Those are T0 (Control), T1 (basal ration with cow's milk), T2 (basal ration with fermented milk), T3 (basal ration with fermented soymilk), T4 (basal ration with fermented milk and fermented soy milk combination in comparison of 1:1) done with four replications. There are 20 experimental units. In each unit, there are five heads with 100 broilers for the 35-day maintenance. Based on the statistical analysis of ANOVA, the result has no significant effect (P>0,05) on cholesterol content of meat. Besides, the result shows that the total of cholesterol content of meat and intestine pH of broiler tend to decrease equal to T0=0.00% (Control), T1= 2.24% (102.55±3,56), T2= 5.42% (99.22±3,06), T3= 2.29% (102.50±3,00), T4= 8.65% (95.83±17,88). The conclusion of this research is that giving of fermented milk, fermented soy milk, and combination of it can decrease meat cholesterol up to 8.65% and intestine pH of broiler.

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Retracted article from publishing in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Aziz Sahin, Zafer Ulutas, Arda Yıldırım, Ertuğrul KUL, Yüksel AKSOY, Emre UĞURLUTEPE Önder SÖZEN, Yusuf KAPLAN

Published in Scientific Papers. Series D. Animal Science, Vol. LIX
Written by Settasit SANGSOPONJIT , Wichai SUPHALUCKSANA

A 2x2 factorial experiment was carried out to determine the effects of two levels of diet supplemented with and without microorganisms in combination with and without sterilized feed on the nutritive value of broiler diets with four replicates in each treatment. Some microorganisms from the gastrointestinal tract of chicken were supplemented in commercial broiler diets. They were bacterial (BC-NA-01), actinomycetes(BI-NA-03, BC-NA-02 and BL-NA-02), Aspergillus niger sp.(BD-PDA-01), Mucor sp.(BL-PDA-02), Rhizopus stolonifer sp.(BI-PDA-02) and Trichoderma sp.(BL-PDA-02). The results of proximate analysis revealed that a diet supplemented with microorganisms had a lower percentage of dry matter and crude fiber in the starter diet(0-3 wks), grower diet(4-5wks) and finisher diet(last period) than the diet without microorganisms (p<0.05). They were higher in the percentage of phosphorus in the starter diet and calcium in both the grower diet and finisher diet than the diet without microorganisms (p<0.05). The sterilized diet had a higher percentage of moisture than the non-sterilized diet (p<0.01). Also, they were higher in the percentage of crude protein and ether extract in the starter diet and crude fiber, calcium in both the grower diet and finisher diet than the non-sterilized diet (p<0.05). The diet supplemented with microorganisms and sterilized was higher in crude protein, ether extract, crude fiber, ash and metabolizable energy than the others in the starter diet (P<0.01). However, crude protein, and metabolizable energy were not significantly different with the control. Also, the crude protein, ash and metabolizable energy were higher than the others except the control in grower and finisher diet (p<0.01).

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